Enhanced glucoamylase production in semi-continuous solid state cultivation of Aspergillus niger NRRL 3122

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Sanzo, Ana V.L. [1 ]
Hasan, Salah D.M. [1 ]
Costa, Jorge A.V. [1 ]
Bertolin, Telma E. [1 ]
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[1] Laboratorio de Engenharia Bioquimica, Fund. Univ. Federal do Rio Grande, P.O. Box 474, Rio Grande, RS 96201-900, Brazil
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Semi-continuous process was used as a alternative type of inoculum during solid state cultivation for glucoamylase production by Aspergillus niger. Two different kinds of inoculum were evaluated: Spores suspended in sterile water and the use of dry fermented bran. Enzyme activity results were statistically evaluated and no significant difference within 95% confidence level was observed for the types of inoculum, allowing the use of dry fermented bran as a good alternative for inoculation. The semi-continuous cultivation leads to a minimum of 30% higher overall yield in comparison with the batch process.
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页码:59 / 62
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