Ectopic expression of human brain derived neurotrophic factor gene in fibroblasts

被引：0
|
作者：
Liu, Z. [1 ]
Chen, J. [1 ]
Wang, R. [1 ]
Xiang, T. [1 ]
Li, X. [1 ]
Lin, J. [1 ]
You, L. [1 ]
Dong, Y. [1 ]
机构：
[1] Laboratory of Surgical Department, First Affiliated Hospital, West China Univ. of Medical Sciences, Chengdu 610041, China
来源：
Shengwu Yixue Gongchengxue Zazhi/Journal of Biomedical Engineering | 2001年 / 18卷 / 03期
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摘要：
The full-length sequence encoding hBDNF was inserted down into the enhancer-promotor regulatory element of Colial to construct minigene Colial1-BDNF. The recombinant eukaryotic expression plasmid pSCEPBFCAT was transfected into human fetal tendon fibroblasts in culture to realize ectopic expression of hBDNF gene in fibroblasts. The results of immuno-dot blot, Elisa and Western hybridization confirm that the expression protein with apparent molecular weight 27 kDa in pSCEPBFCAT transfected fibroblasts possesses antigen activity specific for the antibodies to BDNF.
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页码：408 / 411
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