Ectopic expression of human brain derived neurotrophic factor gene in fibroblasts

被引：0

作者：

Liu, Z. ^{[1
]}

Chen, J. ^{[1
]}

Wang, R. ^{[1
]}

Xiang, T. ^{[1
]}

Li, X. ^{[1
]}

Lin, J. ^{[1
]}

You, L. ^{[1
]}

Dong, Y. ^{[1
]}

机构：

[1] Laboratory of Surgical Department, First Affiliated Hospital, West China Univ. of Medical Sciences, Chengdu 610041, China

来源：

Shengwu Yixue Gongchengxue Zazhi/Journal of Biomedical Engineering | 2001年 / 18卷 / 03期

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摘要：

The full-length sequence encoding hBDNF was inserted down into the enhancer-promotor regulatory element of Colial to construct minigene Colial1-BDNF. The recombinant eukaryotic expression plasmid pSCEPBFCAT was transfected into human fetal tendon fibroblasts in culture to realize ectopic expression of hBDNF gene in fibroblasts. The results of immuno-dot blot, Elisa and Western hybridization confirm that the expression protein with apparent molecular weight 27 kDa in pSCEPBFCAT transfected fibroblasts possesses antigen activity specific for the antibodies to BDNF.

引用

页码：408 / 411

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