Chemical Constituents and Antioxidant Activity of Radix Isatidis

被引：0

作者：

Yan J. ^{[1
]}

Zhao C.-F. ^{[2
]}

Li B.-P. ^{[1
]}

Liu Z.-Q. ^{[3
]}

Guo D.-F. ^{[1
]}

Zheng Z. ^{[3
]}

机构：

[1] Beijing Research Institute of Uranium Geology, Beijing

[2] Jilin University, Changchun

[3] Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun

来源：

| 1600年 / Chinese Society for Mass Spectrometry卷 / 38期

关键词：

Antioxidant activity; Chemical constituents; Electrospray ionization tandem mass spectrometry (ESI-MS/MS); High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS); Radix Isatidis;

D O I：

10.7538/zpxb.youxian.2016.0042

中图分类号：

学科分类号：

摘要：

High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and electrospray ionization tandem mass spectrometry (ESI-MS/MS) were applied to analyze the chemical constituents from acetate, n-BuOH and 95% ethanol extracts of Radix Isatidis. The separation was performed on a Kromasil C18 column (4.6 mm×250 mm×5 μm) with gradient of 0.15% acetic acid/water and methanol as mobile phase with a flow rate of 0.8 mL/min at 25℃. The UV spectra of peaks were obtained by a diode array detector (DAD) under the detection wavelength at 254 nm and 265 nm. In order to get exact data of MS and MS2 spectra, the atmosphere pressure chemical ionization ion source (APCI) and electrospray ionization ion source (ESI) in positive and negative scan mode were utilized. Up to date, twenty-eight compounds are identified from the crude extracts, including indoles, quinazolinones, organic acids, nucleosides, purines, amino acids, flavones and carbohydrates. Meanwhile, the antioxidant activity of the extracts from Radix Isatidis were estimated by the ferric reducing antioxidant power (FRAP) and radical scavenging (DPPH) methods. The results of the antioxidant activities indicated that the compositions of antioxidant activity are mainly existed in the small polarity part. Indoles, quinazolinones, organic acids and porphyrins are the main antioxidative constituents of Radix Isatidis. This method is rapid and sensitive, which can be used for analyzing the chemical compositions and antioxidant activities of traditional Chinese medicine in the future. © 2017, Editorial Board of Journal of Chinese Mass Spectrometry Society. All right reserved.

引用

页码：248 / 255

页数：7

共 16 条

[11] Liu S., Yan J., Li H., Et al., Studies on chemical constituents of compound Indigowoad Root Granule by mass spectrometry, Chemical Journal of Chinese Universities, 31, 6, pp. 1143-1147, (2010)
[12] Lu W.Y., Kimball E., Rabinowitz J.D., A high-performance liquid chromatography-tandem mass spectrometry method for quantitation of nitrogen-containing intracellular metabolites, Journal of the American Society for Mass Spectrometry, 17, 1, pp. 37-50, (2006)
[13] Li R., Fu T., Ji Y., Et al., A study of a Stragalus mongholicus and a Stragalus menbranaceus by high performance liquid chromatography-mass spectrometry, Chinese Journal of Analytical Chemistry, 33, 12, pp. 1676-1680, (2005)
[14] Guo M., Song F., Chen M., Et al., Studies on oligosaccharides from Acanthopanax Senticosus Harms (AS) by electrospray ionization tandem mass spectrometry (MS<sup>n</sup>), Chemical Journal of Chinese Universities, 24, 9, pp. 1577-1581, (2003)
[15] Liu W., Mao Y., Zhong K., Et al., Fast characterization of tobacco leaves by direct electrospray ionization-ion trap mass spectrometry, Chinese Journal of Analysis Laboratory, 27, 2, pp. 65-69, (2008)
[16] Sun M., Qi Y., Song F., Et al., Studies on the isolation and the activitys evaluation of anti-oxidation chemical compositions in erythina variegata, Chinese Archives of Traditional Chinese Medicine, 28, 8, pp. 1762-1765, (2010)

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