One step selective partition of Ε-polylysine present in broth cultures in ionic liquid-based aqueous biphasic systems

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作者
Sequeira, Rosy Alphons [1 ,2 ]
Sharma, Mukesh [1 ,2 ]
Pereira, Matheus M. [3 ]
Singh, Nripat [1 ,2 ]
Bhattacharya, Sourish [4 ]
Chudasama, Nishith A [1 ]
Prasad, Kamalesh [1 ,2 ]
机构
[1] Natural Products & Green Chemistry Division, CSIR-Central Salt & Marine Chemicals Research Institute, Bhavnagar, India
[2] Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India
[3] CICECO - Aveiro Institute of Materials, Department of Chemistry, University of Aveiro, Aveiro, Portugal
[4] Process Design and Engineering Division, CSIR-Central Salt & Marine Chemicals Research Institute, Bhavnagar, India
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Solvent extraction - Amino acids - Chemical stability - Polypropylenes;
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摘要
Four different ionic liquids were synthesized, characterized and used with polypropylene glycol for the formation of aqueous biphasic systems and were used to selectively separate Ε-PL from culture broth. It was observed that the partition of Ε-PL to the IL-rich phase was achieved, and with pure Ε-PL, the extraction efficiency was found to be 99.92% while with culture broth, it was 93.42% for the ABS formed with 2-hydroxyethylammonium formate. Preservation of the chemical and structural stability of Ε-PL in the ABSs indicated the potential of such systems as remarkable platform for the selective separation of Ε-PL from the culture broth. © 2020 Taylor & Francis.
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页码:631 / 639
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