Biodegradable particles for protein delivery: Estimation of the release kinetics inside cells

被引：1
|
作者：
Zyuzin M.V. [1 ]
Hartmann R. [2 ]
Timin A.S. [1 ,3 ]
Carregal-Romero S. [4 ,5 ,6 ]
Parak W.J. [7 ]
Escudero A. [8 ,9 ]
机构：
[1] Department of Physics and Engineering, ITMO University, Lomonosova 9, St. Petersburg
[2] Fachbereich Physik, Philipps Universität Marburg, Marburg
[3] Peter The Great St. Petersburg Polytechnic University, Polytechnicheskaya 29, St. Petersburg
[4] Center for Cooperative Research in Biomaterials (CIC biomaGUNE), Basque Research and Technology Alliance (BRTA), San Sebastián
[5] Ikerbasque, Basque Foundation for Science, Bilbao
[6] CHyN, Universität Hamburg, Hamburg
[7] Departamento de Química Inorgánica, Facultad de Química, Universidad de Sevilla, Calle Profesor García González 1, Seville
[8] Instituto de Investigaciones Químicas (IIQ), Universidad de Sevilla – CSIC, Calle Américo Vespucio 49, Seville
来源：
Biomaterials Advances | 2022年 / 139卷
关键词：
Capsules; Carrier; Drug delivery; Drug release; In vitro; Kinetics; Proteins;
D O I：
10.1016/j.bioadv.2022.212966
中图分类号：
学科分类号：
摘要：
A methodology to quantify the efficiency of the protein loading and in-vitro delivery for biodegradable capsules with different architectures based on polyelectrolytes (dextran sulfate, poly-L-arginine and polyethylenimine) and SiO2 was developed. The capsules were loaded with model proteins such as ovalbumin and green fluorescent protein (GFP), and the protein release profile inside cells (either macrophages or HeLa cells) after endocytosis was analysed. Both, protein loading and release kinetics were evaluated by analysing confocal laser scanning microscopy images using MatLab and CellProfiler software. Our results indicate that silica capsules showed the most efficient release of proteins as cargo molecules within 48 h, as compared to their polymeric counterparts. This developed method for the analysis of the intracellular cargo release kinetics from carrier structures could be used in the future for a better control of drug release profiles. © 2022 Elsevier B.V.
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