Detection of heavy metal lead in lettuce leaves based on fluorescence hyperspectral technology combined with deep learning algorithm

The feasibility analysis of fluorescence hyperspectral imaging technology was studied for the detection of lead content in lettuce leaves. Further, Monte Carlo optimized wavelet transform stacked auto-encoders (WT-MC-SAE) was proposed for dimensionality reduction and depth feature extraction of fluorescence spectral data. The fluorescence hyperspectral images of 2800 lettuce leaf samples were selected and the whole lettuce leaf was used as the region of interest (ROI) to extract the fluorescence spectrum. Five different pre-processing algorithms were used to pre-process the original ROI spectral data including standard normalized variable (SNV), first derivative (1st Der), second derivative (2ndDer), third derivative (3rd Der) and fourth derivative (4th Der). Moreover, wavelet transform stacked auto-encoders (WT-SAE) and WT-MC-SAE were used for data dimensionality reduction, and support vector machine regression (SVR) was used for modeling analysis. Among them, 4th Der tends to be the most useful fluorescence spectral data for Pb content detection at 0.067 ∼ 1.400 mg/kg in lettuce leaves, with Rc2 of 0.9802, RMSEC of 0.02321 mg/kg, Rp2 of 0.9467, RMSEP of 0.04017 mg/kg and RPD of 3.273, and model scale (the number of nodes in the input layer, hidden layer and output layer) was 407-314-286-121-76 under the fifth level of wavelet decomposition. Further studies showed that WT-MC-SAE realizes the depth feature extraction of the fluorescence spectrum, and it is of great significance to use fluorescence hyperspectral imaging to realize the quantitative detection of lead in lettuce leaves. © 2021