Inductive Effect and Mechanism of Rhamnolipid Treatment on Resistance against Black Spot Disease in ‘Zaosu’ Pear Fruit

被引：0
|
作者：
Lu Y. [1 ]
Tan Y. [1 ]
Li Y. [1 ]
Wang X. [1 ]
Bi Y. [1 ]
机构：
[1] College of Food Science and Engineering, Gansu Agricultural University, Lanzhou
来源：
Shipin Kexue/Food Science | 2023年 / 44卷 / 19期
关键词：
Alternaria alternata; induced disease resistance; rhamnolipids; Zaosu’ pear;
D O I：
10.7506/spkx1002-6630-20220512-163
中图分类号：
学科分类号：
摘要：
We performed this study in order to evaluate the control effect and mechanism of the biosurfactant rhamnolipids (RLS) on black spot disease caused by Alternaria alternata in pear fruit. The results showed that RLS treatment at a mass concentration of 20 mg/mL effectively enhanced resistance to A. alternata, and the lesion diameter was only 64.29% of that of the control group at 12 days following the treatment. Furthermore, it was shown that compared to the control group, RLS treatment significantly increased the activities of phenylalanine ammonia lyase (PAL), cinnamate acid 4-hydroxylase (C4H), 4-coumarate coenzyme A ligase (4CL), and cinnamyl alcohol dehydrogenase (CAD), as well as the contents of total phenols and flavonoids in pear fruit tissue. The activities of chitinase and β-1,3-glucanase were significantly induced. The activities of NADPH oxidase (NOX), superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), and the contents of H2O2 and superoxide anion radical in the early and middle stages of storage were also increased by RLS treatment. Meanwhile, the ascorbate-glutathione (ASA-GSH) cycle was activated to maintain the dynamic balance of reactive oxygen species (ROS) production and scavenging in pear fruit tissue, and the membrane permeability and the content of malondialdehyde (MDA) were reduced. Collectively, this study suggested that post-harvest rhamnolipid treatment can enhance black spot disease resistance in pear fruit by regulating phenylpropane metabolism, ROS metabolism and pathogenesis-related proteins. © 2023 Chinese Chamber of Commerce. All rights reserved.
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页码：171 / 179
页数：8
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