Rapid and accurate identification of yeast subspecies by MALDI-MS combined with a cell membrane disruption reagent

被引：0
|
作者：
Zhao, Nan [1 ]
Guo, Wei [2 ]
Li, Jiarui [1 ]
Wang, Hao [3 ]
Guo, Xinhua [1 ,4 ]
机构：
[1] Jilin Univ, Coll Chem, State Key Lab Supramol Struct & Mat, Changchun 130012, Peoples R China
[2] Second Hosp Jilin Univ, Dept Nucl Med, Changchun 130041, Peoples R China
[3] Chinese Acad Sci, Changchun Inst Appl Chem, Key Lab Polymer Ecomat, Changchun 130022, Peoples R China
[4] Jilin Univ, Coll Life Sci, Key Lab Mol Enzymol & Engn, Minist Educ, Changchun 130012, Peoples R China
来源：
FOOD CHEMISTRY | 2024年 / 457卷
基金：
中国国家自然科学基金;
关键词：
Yeast identification; S; cerevisiae; MALDI-MS; Yeast disruption reagent; DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; SACCHAROMYCES-CEREVISIAE; MODELS;
D O I：
10.1016/j.foodchem.2024.140102
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been widely used for microbial analysis. However, due to the impenetrable shell of fungi the direct identification of fungi remains challenges. Targeting on this problem, the yeast Saccharomyces cerevisiae (S. cerevisiae) was selected as a model fungus, and a new fungal cell membrane disruption reagent C18-G1 was used before MALDI-MS detection. As a result, much more intensive peaks which distributed in wider m/z range of S. cerevisiae have been identified in comparison with the use of traditional fungal pretreatment methods. Furthermore, a differential peak at m/z 4993 between two subspecies of S. cerevisiae has been identified. The corresponding protein with exclusive sequence of the specific peak was obtained based on MS/MS fragments and database searching. In addition, the method was successfully applied for the discrimination of four commercial yeasts.
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页数：7
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