Rapid and accurate identification of yeast subspecies by MALDI-MS combined with a cell membrane disruption reagent

被引：0
|
作者：
Zhao, Nan [1 ]
Guo, Wei [2 ]
Li, Jiarui [1 ]
Wang, Hao [3 ]
Guo, Xinhua [1 ,4 ]
机构：
[1] Jilin Univ, Coll Chem, State Key Lab Supramol Struct & Mat, Changchun 130012, Peoples R China
[2] Second Hosp Jilin Univ, Dept Nucl Med, Changchun 130041, Peoples R China
[3] Chinese Acad Sci, Changchun Inst Appl Chem, Key Lab Polymer Ecomat, Changchun 130022, Peoples R China
[4] Jilin Univ, Coll Life Sci, Key Lab Mol Enzymol & Engn, Minist Educ, Changchun 130012, Peoples R China
来源：
FOOD CHEMISTRY | 2024年 / 457卷
基金：
中国国家自然科学基金;
关键词：
Yeast identification; S; cerevisiae; MALDI-MS; Yeast disruption reagent; DESORPTION IONIZATION-TIME; FLIGHT MASS-SPECTROMETRY; SACCHAROMYCES-CEREVISIAE; MODELS;
D O I：
10.1016/j.foodchem.2024.140102
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been widely used for microbial analysis. However, due to the impenetrable shell of fungi the direct identification of fungi remains challenges. Targeting on this problem, the yeast Saccharomyces cerevisiae (S. cerevisiae) was selected as a model fungus, and a new fungal cell membrane disruption reagent C18-G1 was used before MALDI-MS detection. As a result, much more intensive peaks which distributed in wider m/z range of S. cerevisiae have been identified in comparison with the use of traditional fungal pretreatment methods. Furthermore, a differential peak at m/z 4993 between two subspecies of S. cerevisiae has been identified. The corresponding protein with exclusive sequence of the specific peak was obtained based on MS/MS fragments and database searching. In addition, the method was successfully applied for the discrimination of four commercial yeasts.
引用
收藏
页数：7
相关论文
共 37 条
  • [1] Rapid and accurate identification of Streptococcus equi subspecies by MALDI-TOF MS
    Kudirkiene, Egle
    Welker, Martin
    Knudsen, Nanna R.
    Bojesen, Anders M.
    SYSTEMATIC AND APPLIED MICROBIOLOGY, 2015, 38 (05) : 315 - 322
  • [2] Rapid authentication of red wine by MALDI-MS combined with DART-MS
    Lin, Xuewei
    Wu, Hao
    Huang, Gefei
    Wu, Qian
    Yao, Zhong-Ping
    ANALYTICA CHIMICA ACTA, 2023, 1283
  • [3] Top-Down Identification and Sequence Analysis of Small Membrane Proteins Using MALDI-MS/MS
    Meier-Credo, Jakob
    Preiss, Laura
    Wiillenweber, Imke
    Resemann, Anja
    Nordmann, Christoph
    Zabret, Jure
    Suckau, Detlev
    Michel, Hartmut
    Nowaczyk, Marc M.
    Meier, Thomas
    Langer, Julian D.
    JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2022, 33 (07) : 1293 - 1302
  • [4] Lipid Heterogeneity between Astrocytes and Neurons Revealed by Single-Cell MALDI-MS Combined with Immunocytochemical Classification
    Neumann, Elizabeth K.
    Comi, Troy J.
    Rubakhin, Stanislav S.
    Sweedler, Jonathan, V
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2019, 58 (18) : 5910 - 5914
  • [5] Rapid analysis of Gram-positive bacteria in water via membrane filtration coupled with nanoprobe-based MALDI-MS
    Li, Shuping
    Guo, Zhongxian
    Wu, Hui-Fen
    Liu, Ying
    Yang, Zhaoguang
    Woo, Chee Hoe
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2010, 397 (06) : 2465 - 2476
  • [6] Rapid analysis of Gram-positive bacteria in water via membrane filtration coupled with nanoprobe-based MALDI-MS
    Shuping Li
    Zhongxian Guo
    Hui-Fen Wu
    Ying Liu
    Zhaoguang Yang
    Chee Hoe Woo
    Analytical and Bioanalytical Chemistry, 2010, 397 : 2465 - 2476
  • [7] Rapid and accurate identification of genomic species from the Acinetobacter baumannii (Ab) group by MALDI-TOF MS
    Espinal, P.
    Seifert, H.
    Dijkshoorn, L.
    Vila, J.
    Roca, I.
    CLINICAL MICROBIOLOGY AND INFECTION, 2012, 18 (11) : 1097 - 1103
  • [8] Improved MALDI-TOF MS Identification of Mycobacterium tuberculosis by Use of an Enhanced Cell Disruption Protocol
    Bacanelli, Gisele
    Araujo, Flabio Ribeiro
    Verbisck, Newton Valerio
    MICROORGANISMS, 2023, 11 (07)
  • [9] Rapid enrichment of phosphopeptides by BaTiO3 nanoparticles after microwave-assisted tryptic digest of phosphoproteins, and their identification by MALDI-MS
    Suresh Kumar Kailasa
    Hui-Fen Wu
    Microchimica Acta, 2012, 179 : 83 - 90
  • [10] Two-dimensional gel electrophoresis/MALDI-MS followed by iTRAQ™ reagent technology;: A powerful tool in toxicoproteomics for identification and prevalidation of potential early blomarkers
    Kroeger, M.
    Fella, K.
    Glueckmann, M.
    Kruft, V.
    Hellmann, J.
    Kramer, P.
    MOLECULAR & CELLULAR PROTEOMICS, 2005, 4 (08) : S222 - S222
1234