Analysis of Enzyme Conformation Dynamics Using Single-Molecule Förster Resonance Energy Transfer (smFRET)

被引：2
|
作者：
Huynh, Mai [1 ]
Sengupta, Bhaswati [1 ]
机构：
[1] Penn State Univ, Dept Chem, University Pk, PA 16802 USA
来源：
BIOPHYSICA | 2022年 / 2卷 / 02期
关键词：
smFRET; enzymes; conformational dynamics; CRYSTAL-STRUCTURE; FRET METHODS; RNA; FLUORESCENCE; DNA; COMPLEX; CAS9; BINDING; SPECTROSCOPY; RECOGNITION;
D O I：
10.3390/biophysica2020014
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Single-molecule F & ouml;rster resonance energy transfer (smFRET) enables the deconvolution of various conformational substates of biomolecules. Over the past two decades, it has been widely used to understand the conformational dynamics of enzymes. Commonly, enzymes undergo reversible transitions between active and inactive states in solution. Using smFRET, the details of these transitions and the effect of ligands on these dynamics have been determined. In this mini-review, we discuss the various works focused on the investigation of enzyme conformational dynamics using smFRET.
引用
收藏
页码：123 / 134
页数：12
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