The presented study determined the effect of biofertilizers on the growth and production of broad bean (Vicia faba L.) cultivars conducted during the autumn season of 2020-2021 at the Al-Mahaweel area, Babylon Governorate, Iraq. The experiment followed a randomized complete block design with two factors and three replicates. The first factor was the three broad bean cultivars-local, Turkish, and Spanish-while the second was four biofertilizer levels(control, Bacillus mucilaginous, and mycorrhizal fungi). The results showed the Spanish cultivar significantly excelled over other cultivars in yield-related traits, pods per plant (16.82), seeds per pod (6.11), seed yield (5,884.29 kg ha-1), and for all treatments, i.e., pods per plant (17.84), seeds per pod (6.98), seed yield (5,874.11 kg ha-1), and biological yield (14,495.79 kg ha-1). Meanwhile, the interaction treatment (Spanish cultivar + mycorrhiza fungi) superbly shone and gave the highest rate for most studied traits.