A Gold Nanoparticle-Based Cortisol Aptasensor for Non-Invasive Detection of Fish Stress

被引:0
|
作者
Tanaka, Yuki [1 ]
Salleh, Nur Asinah binte Mohamed [1 ]
Tan, Marie Ruoyun [2 ]
Vij, Shubha [2 ,3 ]
Wee, Caroline Lei [4 ]
Sutarlie, Laura [5 ]
Su, Xiaodi [1 ,5 ]
机构
[1] ASTAR, Inst Mat Res & Engn, 2 Fusionopolis Way,Innovis 08-03, Singapore 138634, Singapore
[2] Sch Appl Sci, Republ Polytech, 9 Woodlands Ave 9, Singapore 738964, Singapore
[3] James Cook Univ Singapore, Trop Futures Inst, 149 Sims Dr, Singapore 387380, Singapore
[4] ASTAR, Inst Mol & Cell Biol IMCB, 61 Biopolis Dr, Singapore 138673, Singapore
[5] Natl Univ Singapore, Dept Chem, Block S8,Level 3,3 Sci Dr 3, Singapore 117543, Singapore
关键词
cortisol; aptamer; gold nanoparticle; aptasensor; fish stress; STOCKING DENSITY; LATES-CALCARIFER; APTAMER; BIOSENSOR; QUANTIFICATION; CHEMISTRY; ASSAY;
D O I
10.3390/biom14070818
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cortisol is a key stress biomarker in humans and animals, including fishes. In aquafarming, stress monitoring using cortisol quantification can help to optimize aquaculture practices for welfare and productivity enhancement. However, most current methods for cortisol detection rely on invasive tissue sampling. In this work, we developed a gold nanoparticle (AuNP)-based cortisol sensor to address the demand of detecting picomolar ranges of cortisol from complex fish tank water matrices as a non-invasive alternative for more effective stress monitoring. We first identified a DNA aptamer with effective binding to cortisol and then conjugated the thiol-labelled aptamer to AuNPs together with a blocker molecule (CALNN) to form an Au-Apt-CALNN conjugate that is stable in fish tank water. The cortisol detection principle is based on magnesium chloride (MgCl2)-induced particle aggregation, where the cortisol-bound aptamer on the AuNPs folds into a tertiary structure and provides greater protection for Au-Apt-CALNN against MgCl2-induced aggregation due to steric stabilization. At an optimum MgCl2 concentration, the differential stability of particles with and without cortisol binding offers a limit of detection (LOD) of 100 pM for cortisol within a 35 min reaction. The aptasensor has been validated on recirculating aquaculture system (RAS) fish tank water samples by the HPLC method and was able to detect changes in water cortisol induced by two different stress paradigms. This on-site deployable and non-invasive sensor offers opportunities for more efficient and real-time fish stress monitoring for the optimization of aquaculture practices.
引用
收藏
页数:14
相关论文
共 50 条
  • [21] Development of a label-free gold nanoparticle-based colorimetric aptasensor for detection of human estrogen receptor alpha
    Rajesh Ahirwar
    Pradip Nahar
    [J]. Analytical and Bioanalytical Chemistry, 2016, 408 : 327 - 332
  • [22] Optimization of Gold Nanoparticle-Based DNA Detection for Microarrays
    Grit Festag
    Andrea Steinbrück
    Andreas Wolff
    Andrea Csaki
    Robert Möller
    Wolfgang Fritzsche
    [J]. Journal of Fluorescence, 2005, 15 : 161 - 170
  • [23] Gold nanoparticle-based immunoassay for the detection of bifenthrin in vegetables
    Yao, Jingjing
    Xu, Xinxin
    Liu, Liqiang
    Kuang, Hua
    Xu, Chuanlai
    [J]. FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT, 2022, 39 (03): : 531 - 541
  • [24] Fluorescent Gold Nanoparticle-Based Bioconjugate for the Detection of Salmonella
    Rios-Corripio, M. A.
    Arcila-Lozano, L. S.
    Garcia-Perez, B. E.
    Jaramillo-Flores, M. E.
    Hernandez-Perez, A. D.
    Carlos-Martinez, A.
    Rosales-Perez, M.
    Rojas-Lopez, M.
    [J]. ANALYTICAL LETTERS, 2016, 49 (12) : 1862 - 1873
  • [25] Optimization of gold nanoparticle-based DNA detection for microarrays
    Festag, G
    Steinbrück, A
    Wolff, A
    Csaki, A
    Möller, R
    Fritzsche, W
    [J]. JOURNAL OF FLUORESCENCE, 2005, 15 (02) : 161 - 170
  • [26] Gold nanoparticle-based electrochemical detection of protein phosphorylation
    Kerman, Kagan
    Chikae, Miyuki
    Yamamura, Shohei
    Tamiya, Eiichi
    [J]. ANALYTICA CHIMICA ACTA, 2007, 588 (01) : 26 - 33
  • [27] Gold nanoparticle-based sensing strategies for biomolecular detection
    Wang, Lihua
    Song, Shiping
    Pan, Dun
    Li, Di
    Fan, Chunhai
    [J]. PURE AND APPLIED CHEMISTRY, 2010, 82 (01) : 81 - 89
  • [28] A non-invasive nanoparticle-based sustained dual-drug delivery system as an eyedrop for endophthalmitis
    Mahaling, Binapani
    Baruah, Namrata
    Ahamad, Nadim
    Maisha, Nuzhat
    Lavik, Erin
    Katti, Dhirendra S.
    [J]. INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2021, 606
  • [29] Facemask analyses for the non-invasive detection of chronic and acute P. aeruginosa lung infections using nanoparticle-based immunoassays
    Delgado-Cano, David
    Clemente, Antonio
    Adrover-Jaume, Cristina
    Vaquer, Andreu
    Lopez, Meritxell
    Martinez, Rocio
    Roig, Isabel M.
    Iglesias, Amanda
    Cosio, Borja G.
    de la Rica, Roberto
    [J]. ANALYST, 2023, 148 (19) : 4837 - 4843
  • [30] Optimisation of a gold nanoparticle-based aptasensor integrated with image processing for the colorimetric detection of acephate using response surface methodology
    Osman, Mohd Junaedy
    Rashid, Jahwarhar Izuan Abdul
    Khim, Ong Keat
    Yunus, Wan Md Zin Wan
    Noor, Siti Aminah Mohd
    Kasim, Noor Azilah Mohd
    Knight, Victor Feizal
    Chuang, Teoh Chin
    [J]. RSC ADVANCES, 2021, 11 (42) : 25933 - 25942