A Gold Nanoparticle-Based Cortisol Aptasensor for Non-Invasive Detection of Fish Stress

被引:0
|
作者
Tanaka, Yuki [1 ]
Salleh, Nur Asinah binte Mohamed [1 ]
Tan, Marie Ruoyun [2 ]
Vij, Shubha [2 ,3 ]
Wee, Caroline Lei [4 ]
Sutarlie, Laura [5 ]
Su, Xiaodi [1 ,5 ]
机构
[1] ASTAR, Inst Mat Res & Engn, 2 Fusionopolis Way,Innovis 08-03, Singapore 138634, Singapore
[2] Sch Appl Sci, Republ Polytech, 9 Woodlands Ave 9, Singapore 738964, Singapore
[3] James Cook Univ Singapore, Trop Futures Inst, 149 Sims Dr, Singapore 387380, Singapore
[4] ASTAR, Inst Mol & Cell Biol IMCB, 61 Biopolis Dr, Singapore 138673, Singapore
[5] Natl Univ Singapore, Dept Chem, Block S8,Level 3,3 Sci Dr 3, Singapore 117543, Singapore
关键词
cortisol; aptamer; gold nanoparticle; aptasensor; fish stress; STOCKING DENSITY; LATES-CALCARIFER; APTAMER; BIOSENSOR; QUANTIFICATION; CHEMISTRY; ASSAY;
D O I
10.3390/biom14070818
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Cortisol is a key stress biomarker in humans and animals, including fishes. In aquafarming, stress monitoring using cortisol quantification can help to optimize aquaculture practices for welfare and productivity enhancement. However, most current methods for cortisol detection rely on invasive tissue sampling. In this work, we developed a gold nanoparticle (AuNP)-based cortisol sensor to address the demand of detecting picomolar ranges of cortisol from complex fish tank water matrices as a non-invasive alternative for more effective stress monitoring. We first identified a DNA aptamer with effective binding to cortisol and then conjugated the thiol-labelled aptamer to AuNPs together with a blocker molecule (CALNN) to form an Au-Apt-CALNN conjugate that is stable in fish tank water. The cortisol detection principle is based on magnesium chloride (MgCl2)-induced particle aggregation, where the cortisol-bound aptamer on the AuNPs folds into a tertiary structure and provides greater protection for Au-Apt-CALNN against MgCl2-induced aggregation due to steric stabilization. At an optimum MgCl2 concentration, the differential stability of particles with and without cortisol binding offers a limit of detection (LOD) of 100 pM for cortisol within a 35 min reaction. The aptasensor has been validated on recirculating aquaculture system (RAS) fish tank water samples by the HPLC method and was able to detect changes in water cortisol induced by two different stress paradigms. This on-site deployable and non-invasive sensor offers opportunities for more efficient and real-time fish stress monitoring for the optimization of aquaculture practices.
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页数:14
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