Coating of liposomes with β-glucans from the king oyster mushroom ( Pleurotus eryngii)

被引:0
|
作者
de Carvalho, Mariana Mazetto [1 ]
Eltvik, Andrea Angelov [1 ]
Ellefsen, Christiane Faerestrand [1 ]
Hiorth, Marianne [1 ]
Samuelsen, Anne Berit C. [1 ]
机构
[1] Univ Oslo, Dept Pharm, POB 1068 Blindern, N-136 Oslo, Norway
关键词
M ushroom beta-glucans; Liposomes; Polymer -coated liposomes; CHAIN CONFORMATION; BRANCHED (1; POLYSACCHARIDE; STABILITY; POLYMERS; DELIVERY;
D O I
10.1016/j.colsurfa.2024.134783
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The aim of this study was to investigate the ability to coat liposomes with 13-glucans from Pleurotus eryngii, in order to obtain a suitable formulation for pharmaceutical purposes. After alkali extraction of the fruiting bodies of P. eryngii and solvent fractionation of the raw polysaccharide material, four polysaccharide fractions named PEA2-SS, PEA2-SP, PEA3-SS and PEA3-SP were obtained. Chemical structure analyses by methanolysis, methylation and NMR indicated that all fractions contained two types of 13-glucans: one 3-linked with some branching at O-6 and one 6-linked with branches occurring at O-3. The molar mass ranged from 7 - 36 kDa. Fraction PEA2-SP was most suitable for the coating process according to the resulting charge, hydrodynamic diameter (size) and the PDI of this formulation. This was attributed to the higher Mw displayed by this fraction. After enzymatic degradation, the ability of PEA2-SP to coat the liposomes was lost, indicating the requirement of a minimum polymer molar mass, which is suggested to be around 30 kDa. Furthermore, the presence of a positive charge on the liposome surface seemed to be essential. The novel 13-glucans coated liposomes were able to activate dectin-1b, a receptor on macrophages, and may have potential as a drug delivery platform.
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页数:13
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