A new exploration of the amplification mechanism of saltatory rolling circle amplification (SRCA) for food safety detection

A new property of Bst DNA polymerase was discovered by our team, which led to the establishment of a new isothermal amplification method for SRCA nucleic acid, which was applied to food safety detection, investigated the amplification mechanism, and the SRCA version 1.0 amplification mechanism was proposed. This study proposes a new SRCA amplification mechanism (version 2.0) by using the autosynthetic sequences CDG2040 and CDG2068, as well as the Listeria monocytogenes hlyA gene and Vibrio parahaemolyticus toxR gene as target sequences to further investigate the amplification mechanism. The initiation of the SRCA reaction depends primarily on the spatial structure of the non-closed circle formed by the discontinuous complementation of the bases on both sides of the target sequence. The DNA fragments that connect the target sequences in the SRCA reaction are the sum of the complementary sequences of adjacent partial sequences on both sides of the target sequence.