Synthesis and Characterization of Novel Chitosan-Nickel oxide Based Amygdalin Hybrid Nanomaterials for Antibacterial and Anticancer

Background: Nanotechnology has made tremendous progress in drug development and delivery. The functionalization of nanoparticles with various biological agents facilitates the drug delivery and treatment of various cancers. Objectives: The current work was conducted to synthesize the chitosan-nickel oxide-amygdalin hybrid nanomaterials (Chitosan-NiO-Amygdalin HNMs) and evaluate their antimicrobial and cytotoxic effects on liver cancer (Hep3B) and breast cancer (MCF-7) cells. Materials and Methods: The synthesized Chitosan-NiO-Amygdalin HNMs were characterized using various methods, including UV-visible spectroscopy, PL, XRD, DLS, SEM, TEM and FT-IR analyses. The antibacterial property of Chitosan-NiO-Amygdalin HNMs was assessed by the disc diffusion method against various pathogens. The cytotoxicity of Chitosan-NiOAmygdalin HNMs on Hep3B and MCF-7 cells was assessed by an MTT assay. Results:The synthesis of Chitosan-NiO-Amygdalin HNMs was confirmed by a UV-vis spectrophotometry study. The PL emission spectrum of the Chitosan-NiO-Amygdalin HNMs shows their optoelectronic properties. The XRD patterns reveal that the HNMs possess a cubic crystal structure. The SEM images showed that the HNMs exhibit hexagonal structures. TEM images illustrate HNMs with hexagonal morphology. The HNMs have also shown potential antibacterial effects against various pathogens. The Chitosan-NiO-Amygdalin HNMs effectively inhibited the viability of both Hep3B and MCF-7 cells. Conclusion: The results of this work suggest that Chitosan-NiO-Amygdalin HNMs exhibit potential to be a prospective therapeutic agent in the future to treat cancer and other diseases.