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Toward DNA-Based Recording of Biological Processes
被引:0
|作者:
Jang, Hyeri
[1
]
Yim, Sung Sun
[1
,2
,3
,4
]
机构:
[1] Korea Adv Inst Sci & Technol KAIST, Dept Biol Sci, Daejeon 34141, South Korea
[2] Korea Adv Inst Sci & Technol KAIST, Grad Sch Engn Biol, Daejeon 34141, South Korea
[3] KAIST Korea Adv Inst Sci & Technol, KAIST Inst BioCentury, Daejeon 34141, South Korea
[4] Korea Res Inst Biosci & Biotechnol KRIBB, Daejeon 34141, South Korea
基金:
新加坡国家研究基金会;
关键词:
DNA-based data storage;
molecular recording;
cellular memory;
synthetic biology;
GENE-EXPRESSION;
IN-VIVO;
GENOMIC DNA;
MEMORY;
METHYLATION;
EFFICIENT;
INFORMATION;
CIRCUITS;
CELLS;
TIME;
D O I:
10.3390/ijms25179233
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Exploiting the inherent compatibility of DNA-based data storage with living cells, various cellular recording approaches have been developed for recording and retrieving biologically relevant signals in otherwise inaccessible locations, such as inside the body. This review provides an overview of the current state of engineered cellular memory systems, highlighting their design principles, advantages, and limitations. We examine various technologies, including CRISPR-Cas systems, recombinases, retrons, and DNA methylation, that enable these recording systems. Additionally, we discuss potential strategies for improving recording accuracy, scalability, and durability to address current limitations in the field. This emerging modality of biological measurement will be key to gaining novel insights into diverse biological processes and fostering the development of various biotechnological applications, from environmental sensing to disease monitoring and beyond.
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页数:17
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