Deciphering nanoparticle protein coronas by capillary isoelectric focusing-mass spectrometry-based top-down proteomics

被引：2
|
作者：
Zhu, Guijie [1 ]
Sadeghi, Seyed Amirhossein [1 ]
Mahmoudi, Morteza [2 ]
Sun, Liangliang [1 ]
机构：
[1] Michigan State Univ, Dept Chem, 578 S Shaw Lane, E Lansing, MI 48824 USA
[2] Michigan State Univ, Dept Radiol & Precis Hlth Program, E Lansing, MI 48824 USA
来源：
CHEMICAL COMMUNICATIONS | 2024年 / 60卷 / 81期
基金：
美国国家科学基金会;
关键词：
SEPARATION; RESOLUTION;
D O I：
10.1039/d4cc02666g
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The nanoparticle (NP) protein corona significantly influences the outcome of nanomedicine. We present the first example of top-down proteomics (TDP) measurement of the protein corona using capillary isoelectric focusing-mass spectrometry, identifying seventy proteoforms of 16 cancer-related genes. This technique has the potential to revolutionize our understanding of the protein corona and advance nanomedicine. The first example of top-down proteomics of nanoparticle protein coronas using capillary isoelectric focusing-mass spectrometry.
引用
收藏
页码：11528 / 11531
页数：4
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