High-Resolution Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy of Paired Clinical Liver Tissue Samples from Hepatocellular Cancer and Surrounding Region

被引:0
|
作者
Fernandes, Wendy M. [1 ,2 ]
Harris, Nicola [1 ,2 ]
Zamalloa, Ane [3 ]
Adofina, Lissette [3 ]
Srinivasan, Parthi [3 ]
Menon, Krishna [3 ]
Heaton, Nigel [3 ]
Miquel, Rosa [4 ]
Zen, Yoh [4 ]
Kelly, Geoff [5 ]
Jarvis, James A. [6 ,7 ]
Oregioni, Alain [5 ]
Chokshi, Shilpa [1 ,2 ]
Riva, Antonio [1 ,2 ]
Cox, I. Jane [1 ,2 ]
机构
[1] Fdn Liver Res, Roger Williams Inst Hepatol, 111 Coldharbour Lane, London SE5 9NT, England
[2] Kings Coll London, Fac Life Sci & Med, London WC2R 2LS, England
[3] Kings Coll Hosp NHS Fdn Trust, Inst Liver Studies, Denmark Hill, London SE5 9RS, England
[4] Kings Coll Hosp NHS Fdn Trust, Inst Liver Studies, Liver Histopathol Lab, Denmark Hill, London SE5 9RS, England
[5] Francis Crick Inst, MRC Biomed NMR Ctr, 1 Midland Rd, London NW1 1AT, England
[6] Kings Coll London, Randall Ctr Cell & Mol Biophys, London SE1 1UL, England
[7] Kings Coll London, Ctr Biomol Spect, London SE1 1UL, England
基金
英国惠康基金; 英国医学研究理事会;
关键词
hepatocellular carcinoma; NMR spectroscopy; lipid metabolism; bacterial metabolites; personalised treatment; MAS-NMR-SPECTROSCOPY; METABOLIC PROFILE; DISEASE; CARCINOMA; EFFICACY; GLUCOSE; IMPACT; DONOR;
D O I
10.3390/ijms25168924
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The global burden of liver cancer is increasing. Timely diagnosis is important for optimising the limited available treatment options. Understanding the metabolic consequences of hepatocellular carcinoma (HCC) may lead to more effective treatment options. We aimed to document metabolite differences between HCC and matched surrounding tissues of varying aetiology, obtained at the time of liver resection, and to interpret metabolite changes with clinical findings. High-resolution magic angle spinning nuclear magnetic resonance (HRMAS-NMR) spectroscopy analyses of N = 10 paired HCC and surrounding non-tumour liver tissue samples were undertaken. There were marked HRMAS-NMR differences in lipid levels in HCC tissue compared to matched surrounding tissue and more subtle changes in low-molecular-weight metabolites, particularly when adjusting for patient-specific variability. Differences in lipid-CH3, lipid-CH2, formate, and acetate levels were of particular interest. The obvious differences in lipid content highlight the intricate interplay between metabolic adaptations and cancer cell survival in the complex microenvironment of liver cancer. Differences in formate and acetate might relate to bacterial metabolites. Therefore, documentation of metabolites in HCC tissue according to histology findings in patients is of interest for personalised medicine approaches and for tailoring targeted treatment strategies.
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页数:19
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