Proteomic analysis of two novel peptides from the Odontobuthus doriae scorpion venom

被引：0

作者：

Zargan, Jamil ^{[1
]}

Jahangirian, Ehsan ^{[1
]}

Khan, Haider A. ^{[2
]}

Ali, Shakir ^{[3
]}

机构：

[1] Imam Hossein Univ, Fac Basic Sci, Dept Biol, Nanobiotechnol, Tehran 1698715461, Iran

[2] Hamdard Univ, Dept Med Elementol & Toxicol, Jamia Hamdard, New Delhi 110062, India

[3] Hamdard Univ, Dept Biochem, Jamia Hamdard, New Delhi 110062, India

来源：

JOURNAL OF ASIAN NATURAL PRODUCTS RESEARCH | 2025年 / 27卷 / 03期

关键词：

Odontobuthus doriae; MALDI-TOF/MS; scorpion venom; proteomic; peptide; LEUKEMIA-CELL LINE; MOLECULAR CHARACTERIZATION; SELECTIVE TOXIN; PROTEINS; PURIFICATION; APOPTOSIS; CHLOROTOXIN; COMPONENTS; FRACTIONS; CHANNELS;

D O I：

10.1080/10286020.2024.2403612

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The venom of the Odontobuthus doriae scorpion, prevalent in East Asia and Iran, has not been fully characterized. This study provides the first proteomic profile of O. doriae venom to explore its potential as a medical. 2D-PAGE analysis revealed 96 protein spots with isoelectric points from 3 to 9 and molecular weights between 6.6 to 205 kDa. Fourteen toxin fractions were isolated via HPLC, and SDS-PAGE showed seven protein bands ranging from 3.8 to 182 kDa. MALDI-TOF MS identified Peptide 1 and Peptide 2, resembling Hemoglobin beta-2 chain and Chaperonin HSP60 and suggest potential therapeutic applications for P1 and P2.

引用

页码：301 / 322

页数：22

共 50 条

[31] Molecular dissection of venom from Chinese scorpion Mesobuthus martensii:: Identification and characterization of four novel disulfide-bridged venom peptides
Zeng, Xian-Chun
Luo, Feng
Li, Wen-Xin
PEPTIDES, 2006, 27 (07) : 1745 - 1754
[32] OdK2, a Kv1.3 channel-selective toxin from the venom of the Iranian scorpion Odonthobuthus doriae
Abdel-Mottaleb, Yousra
Vandendriessche, Thomas
Clynen, Elke
Landuyt, Bart
Jalali, Amir
Vatanpour, Hossein
Schoofs, Liliane
Tytgat, Jan
TOXICON, 2008, 51 (08) : 1424 - 1430
[33] Structure, function and mechanistic aspects of scorpion venom peptides - A boon for the development of novel therapeutics
Suhas, R.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY REPORTS, 2022, 6
[34] Genomic organization of BmTXKβ and BmTXKS2, two scorpion venom peptides from Buthus martensii karsch
Cao, ZJ
Zhu, SY
Li, WX
Jiang, DH
He, XH
Liu, H
IUBMB LIFE, 2001, 51 (05) : 305 - 308
[35] Proteomic analysis and lethality of the venom of Aegaeobuthus nigrocinctus, a scorpion of medical significance in the Middle East
Borges, Adolfo
Lomonte, Bruno
ACTA TROPICA, 2024, 255
[36] CHARACTERIZATION OF INSECTICIDAL PEPTIDES FROM THE VENOM OF THE NORTH AFRICAN SCORPION, BUTHACUS LEPTOCHELYS
Yoshimoto, Yusuke
Miyashita, Masahiro
Abdel-Wahab, Mohammed
Sarhan, Moustafa
Nakagawa, Yoshiaki
Miyagawa, Hisashi
TOXICON, 2020, 177 : S62 - S62
[37] Characterization of venom components from the scorpion Androctonus crassicauda of Turkey:: Peptides and genes
Caliskan, Figen
Garcia, Blanca Ines
Coronas, Fredy I. V.
Batista, Cesar V. F.
Zamudio, Fernando Z.
Possani, Lourival D.
TOXICON, 2006, 48 (01) : 12 - 22
[38] Characterization of a novel cDNA encoding a short venom peptide derived from venom gland of scorpion Buthus martensii Karsch:: Trans-splicing may play an important role in the diversification of scorpion venom peptides
Zeng, XC
Luo, F
Li, WX
PEPTIDES, 2006, 27 (04) : 675 - 681
[39] POTASSIUM CHANNEL BLOCKER PEPTIDES ISOLATED FROM VENOM OF THE SCORPION BUTHOTUS SCHACH
Aboutorabi, Akram
Naderi, Nima
Gholamipour-Bade, Hamid
Motamedi, Fereshteh
Zolfagharian, Hossein
Vatanpour, Hossein
TOXICON, 2019, 158 : S53 - S54
[40] Characterization of Sodium Channel Peptides Obtained from the Venom of the Scorpion Centruroides bonito
Restano-Cassulini, Rita
Olamendi-Portugal, Timoteo
Riano-Umbarila, Lidia
Zamudio, Fernando Z.
Delgado-Prudencio, Gustavo
Becerril, Baltazar
Possani, Lourival D.
TOXINS, 2024, 16 (03)

← 1 2 3 4 5 →