Phase separation of triton X-100 and bovine serum albumin mixture: Impacts of nature and composition of polyols on associated physicochemical parameters

Bovine serum albumin (BSA) is widely used in tissue engineering and pharmaceutical research. It is readily available as a byproduct of the cattle industry, and collected from blood. In this study, we conducted a physicochemical investigation of the phase separation in a mixture of Triton X-100 (TX-100) and BSA, influenced by various polyols, using the well-established cloud point (CP) determination method. The addition of polyols resulted in a significant reduction in CP values for the TX-100 + BSA mixture. The magnitudes of CP in the experimental system were highly varied with different polyols and followed the order of: CP H2O (+Xylose) > CPH2O (+Mannose) > CP H2O (+Arabinose) > CP H2O (+Fructose) > CP H2O (+Dextrsoe) > CPH2O (+Glucose) > CP H2O (+Maltose). Under identical conditions, the system exhibited maximum solubility in the xylose solution and minimum solubility in the maltose solution. The positive Delta G(c)(0) values were acquired in all working medium imply the nonspontaneity of phase transition in the TX-100 + BSA system. At lower polyol contents, the negative values of standard enthalpy (Delta H-c(0 )) and standard entropy (Delta S-c(0)) changes were observed, suggesting that electrostatic forces dominated as the driving force for clouding. At highest employed polyols concentration in some case, the positive values for Delta H(c)(0 )and Delta S-c(0) were achieved, which indicated that hydrophobic interactions likely dominate the phase partitioning of the amphiphile and protein mixture. Additionally, entropy-enthalpy compensation parameters were calculated and analyzed with a rational approach. Molecular docking analysis further demonstrated the presence of hydrogen bonds and hydrophobic interactions between TX-100 and BSA.