Protocol for the generation of cultured cortical brain organoid slices

被引：1

作者：

Petersilie, Laura ^{[1
]}

Kafitz, Karl W. ^{[1
]}

Neu, Louis A. ^{[1
]}

Heiduschka, Sonja ^{[2
,3
]}

Le, Stephanie ^{[2
,3
]}

Prigione, Alessandro ^{[2
,3
]}

Rose, Christine R. ^{[1
]}

机构：

[1] Heinrich Heine Univ Duesseldorf, Fac Math & Nat Sci, Inst Neurobiol, D-40225 Dusseldorf, Germany

[2] Univ Childrens Hosp, Dept Gen Pediat Neonatol & Pediat Cardiol, D-40225 Dusseldorf, Germany

[3] Heinrich Heine Univ Duesseldorf, D-40225 Dusseldorf, Germany

来源：

STAR PROTOCOLS | 2024年 / 5卷 / 03期

关键词：

D O I：

10.1016/j.xpro.2024.103212

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Three-dimensional brain organoids from human pluripotent stem cells are a powerful tool for studying human neural networks. Here, we present a protocol for generating cortical brain organoid slices (cBOS) derived from regionalized cortical organoids and grown at the air-liquid interphase. We provide steps for slicing organoids and maintaining them in long-term culture. We then detail approaches for quality control including the evaluation of cell death and cellular identity. Finally, we describe procedures for the expression of a genetically encoded nanosensor for ATP. For complete details on the use and execution of this protocol, please refer to Petersilie et al.1 1

引用

页数：15

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