New spectrophotometric method for determination of cefepime in pure and pharmaceutical formulation by cloud point extraction in trace

New simple sensitive spectrophotometric methods are developed for determination of cefepime (CFM) in pure and Pharmaceutical Formulations. The first method conversion primary amine to azo-dye by react cefepime with sodium nitrite and hydrochloric acid followed by coupling with 3-aminophenol in alkaline medium to obtain a stable orange colored dye at 2 max 495 nm. The concentration ranges 2-50 mu g / mL, Beer's law is obeyed, correlation coefficient was 0.9997, molar absorptivity was 0.704 x 104L.mol- 1.cm- 1 and the detection limit was 0.259 mu g/mL. Cloud point extraction (CPE) second method to estimation a trace amount in aqueous solution product from diazotization and measuring with a UV-visible spectrophotometer at 2 max 495 nm. The concentration range obeyed the Beer's law was 0.25-10 mu g / mL, correlation coefficient was 0.9996, molar absorptivity was 1.362 x 105L.mol- 1.cm- 1, detection limit was 0.013 mu g/mL, Pre-concentration factor was 25 and Distribution coefficient(D) was 333.35.