Preparation and characterization of electrospun apigenin-loaded polycaprolactone nanofibers for wound dressing applications

Objective(s): This study uses the blend-electrospinning method to explore the development of apigenin) APG(-loaded PCL nanofibers as a promising wound dressing material. Materials and Methods: The approach combines APG's anti-inflammatory and antioxidant properties with the advantages of nanofibers for wound healing. The research investigates the electrospinning process for optimal parameters and characterizes the resulting nanofibers using FE-SEM, FTIR, and contact angle measurements. Results: The findings demonstrate successful APG incorporation into PCL nanofibers at concentrations up to 0.5 wt%. The APG release profile indicates a sustained release over 48 hours. Biocompatibility and cytotoxicity assessments using the Alamar Blue assay reveal excellent biocompatibility of APG-loaded PCL nanofibers (over 90% viability). Additionally, the nanofibers exhibit a porous, bead-free structure with improved hydrophilicity due to APG incorporation. Conclusion: Overall, this study highlights the development of APG/PCL nanofibers with promising characteristics for wound dressing applications. The combination of APG's therapeutic properties, sustained release profile, and biocompatible nanofiber structure suggests their potential for effective wound healing.