Vagococcus jeotgali sp. nov., isolated from cutlassfish jeotgal, a traditional Korean fermented seafood

A novel, Gram- positive, facultatively anaerobic, and non- motile bacterial strain, designated B2T- 5T, T , was isolated from jeotgal, a traditional Korean fermented seafood. Colonies grown on gifu anaerobic medium agar plates were cream- coloured, irregular, and umbonate with curled margins. Optimal growth of strain B2T- 5T T occurred at 20 degrees C, pH 8.0, and in the presence of 1% (w/v) NaCl. Strain B2T- 5T T was negative for oxidase and catalase activity. Hippurate was not hydrolysed and acetoin was not produced. The major cellular fatty acids were C 18: 1 omega 9 c and C 16 :0. . The cell- wall peptidoglycan was of the A4 alpha alpha type containing l-Lys-D- Asp. The predominant respiratory quinone was menaquinone 7. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylcholine. According to the phylogenetic analysis based on 16S rRNA gene sequences, strain B2T- 5T T was most closely related to Vagococcus teuberi DSM 21459T, T , showing 98.2% sequence similarity. Genome sequencing of strain B2T- 5T T revealed a genome size of 2.0 Mbp and a G+C content of 33.8 mol%. The average nucleotide identities of strain B2T- 5T T with Vagococcus teuberi DSM 21459T, T , Vagococcus bubulae SS1994T, T , and Vagococcus martis D7T301T T were 75.0, 74.7, and 75.1%, respectively. Based on the phenotypic, chemotaxonomic, and genotypic data, strain B2T- 5T T represents a novel species of the genus Vagococcus, , for which the name Vagococcus jeotgali sp. nov. is proposed. The type strain is B2T- 5T T (=KCTC 21223T=JCM T =JCM 35937T). T ).