Simultaneous determination of cucurbitacins B, D, and E in Cucurbita seed oils by HPLC-DAD

被引:0
|
作者
Goya-Jorge, Elizabeth [1 ,2 ]
Jorge-Rodriguez, Maria Elisa [1 ]
Saucedo-Hernandez, Yanelis [1 ]
机构
[1] Univ Cent Marta Abreu Villas, Fac Chem & Pharm, Dept Pharm, Lab Pharmaceut Anal, Santa Clara, Cuba
[2] North Carolina State Univ, Coll Vet Med, Dept Clin Sci, Intestinal Regenerat Med Lab, Raleigh, NC USA
关键词
Cucurbitacin B; cucurbitacin D; cucurbitacin E; oilseeds; cucurbit; Cucurbita genus; triterpenoids; RAT PLASMA;
D O I
10.1080/14786419.2024.2394097
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Cucurbitacins are triterpene bioactive constituents of natural products, particularly in the Cucurbitaceae plant family. The presence of cucurbitacins in seeds of the Cucurbita genus (pumpkin) has been only little studied. In this work, the content of cucurbitacins B, D, and E in seed oils from three cucurbits (Cucurbita moschata Duch, Cucurbita pepo Linn, and Cucurbita maxima Linn) was studied. An analytical method based on HPLC-DAD for the detection and quantification of these three cucurbitacins in seed oils was developed and validated according to ICH guidelines. The method showed good linearity, accuracy, and precision for the simultaneous quantification of cucurbitacins B, D, and E using C.moschata seed oil as a reference. When applied to C.pepo and C.maxima seed oils, cucurbitacin B and D were quantified but to a lesser extent. This is the first report of a simple, repeatable, and reproducible analytical tool to identify cucurbitacins in oilseeds from Cucurbita spp. [GRAPHICS] .
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页数:7
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