ROS-Induced Autophagy of Skeletal Muscle Confers Resistance of Rice Flower Carp (Cyprinus carpio) to Short-Term Fasting

被引:1
|
作者
Cheng, Jia [1 ,2 ]
Luo, Junhan [2 ]
Xu, Ziyang [2 ]
Liu, Zhouying [2 ]
Bao, Lingsheng [2 ]
Xue, Liangyi [1 ]
机构
[1] Ningbo Univ, Sch Marine Sci, Ningbo 315832, Peoples R China
[2] Changsha Univ, Coll Biol & Chem Engn, Hunan Engn Technol Res Ctr Amphibian & Reptile Res, Changsha 410022, Peoples R China
基金
中国国家自然科学基金;
关键词
short-term fasting; autophagy; skeletal muscle; rice flower carp; oxidative stress; OXIDATIVE STRESS; STARVATION; DISEASE; CELLS;
D O I
10.3390/genes15070840
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Starvation is one of the main stresses for fish due to food shortage, the evasion of predators, and intraspecific competition. This research evaluated the impact of brief fasting periods on reactive oxygen species (ROS) levels, antioxidant response, mRNA expression of antioxidants, autophagy-related signaling genes, and autophagosome development in the muscle tissue of rice flower carp. Following a three-day fasting period, the levels of ROS and MDA rose. Additionally, after 3 d of fasting, there was a notable upregulation of NRF2 and significant increases in the levels of GSH and the activities of enzymes such as SOD, CAT, GST, GR, and GPX, while the expression of the autophagy marker gene LC3B did not change (p < 0.05). After 7 d of fasting, the content of the ROS, the activity of SOD and GR, and the GSH content reached the maximum (p < 0.05). Concurrently, there was a significant rise in the quantity of autophagosomes. An RT-qPCR analysis revealed that seven d of starvation significantly elevated the mRNA expression of genes associated with the initiation and expansion of autophagosome membranes, vesicle recycling, and cargo recruitment, including ULK1, BECLIN1, LC3B, ATG3, ATG4B, ATG4C, ATG5, ATG9, and P62. After feeding resumed for 3 d, the mRNA level of BECLIN1, ATG3, ATG4B, ATG4C, ATG5, LC3B, and P62 still remained at a high level. The LC3II protein reached its highest level. All autophagy-related gene expression decreased in the 7-day resumed feeding group. Our data implied that short-term fasting can cause oxidative stress and disrupt the antioxidant system first and then induce autophagy in the muscles of rice flower carp. These findings shed light on how fasting affects muscle homeostasis in fish. ROS-induced autophagy of the skeletal muscle may confer the resistance of rice flower carp to short-term fasting.
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页数:11
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