Closed-tube saltatory rolling circle amplification assay for rapid and visual detection of allergenic cashew nut in foods

被引：1

作者：

Chen, Yingying ^{[1
]}

Guo, Zipeng ^{[1
]}

Sun, Zhaoqing ^{[1
]}

Wu, Xinyu ^{[1
]}

Xu, Yifan ^{[1
]}

Jin, Zhengye ^{[1
]}

Cheng, Yuying ^{[1
]}

She, Zhen ^{[1
]}

Jiang, Qianqian ^{[1
]}

Wo, Yuxin ^{[1
]}

Wu, Ting ^{[1
]}

Liu, Hongwang ^{[1
]}

Liu, Meihui ^{[1
]}

Liu, Yulu ^{[1
]}

Diao, Enjie ^{[1
]}

Li, Dengchao ^{[1
]}

Mao, Ruifeng ^{[1
]}

机构：

[1] Huaiyin Normal Univ, Sch Life Sci, Jiangsu Key Lab Food Safety & Nutr Funct Evaluat, Huaian 223300, Jiangsu, Peoples R China

来源：

FOOD CONTROL | 2024年 / 166卷

基金：

中国国家自然科学基金;

关键词：

Food allergy; Allergen detection; Isothermal assay; Anacardium occidentale L; MEDIATED ISOTHERMAL AMPLIFICATION; DNA;

D O I：

10.1016/j.foodcont.2024.110769

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Cashew nut allergy shows an increasing prevalence along with its high consumption. Nowadays, food allergy still has no cure, and allergen avoidance is still the standard management, urging a sensitive and convenient cashew nut detection method. Herein, saltatory rolling circle amplification (SRCA), loop-mediated isothermal amplification (LAMP), as well as polymerase chain reaction (PCR) targeting the Ana o 2 gene were proposed and compared. In addition, a closed-tube SRCA assay using hydroxynaphthol blue (HNB) as a color indicator (HNBSRCA) was developed to realize visual on-site detection of cashew nut. SRCA and HNB-SRCA can detect 75.3 fg of cashew nut DNA, which is 10- and 100-times more sensitive than LAMP and PCR, respectively. SRCA and HNBSRCA exhibited a relative sensitivity of 0.01% in a binary mixture, which is comparable to LAMP and 10-times higher than that of PCR. When combined with a rapid DNA extraction method, SRCA and HNB-SRCA performed better applicability compared to LAMP and PCR. Additionally, compared to SRCA using electrophoresis to analyze the amplification products, the visual closed-tube HNB-SRCA combined with the rapid DNA extraction protocol is a better potential cashew nut on-site detection strategy in foods.

引用

页数：10

共 43 条

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