Microbacterium humicola sp. nov., Microbacterium terrisoli sp. nov., Paenibacillus pedocola sp. nov., Paenibacillus silviterrae sp. nov., Flavobacterium terrisoli sp. nov., and Aquabacterium humicola sp. nov., isolated from soil

Four Gram-stain-positive and two Gram-stain-negative bacterial strains, designated as W4(T), FW7(T), TW48(T), UW52(T), PT-3(T), and RJY3(T), were isolated from soil samples collected from the Republic of Korea. The 16S rRNA gene sequence analysis showed that strains W4(T) and FW7(T) belonged to the genus Microbacterium, strains TW48(T) and UW52(T) were affiliated to the genus Paenibacillus, strain PT-3(T) was related to the genus Flavobacterium, and strain RJY3(T) was associated with the genus Aquabacterium. The closest phylogenetic taxa to W4(T), FW7(T), TW48(T), UW52(T), PT-3(T), and RJY3(T) were Microbacterium bovistercoris NEAU-LLET (97.7 %), Microbacterium protaetiae DFW100M-13(T) (97.9 %), Paenibacillus auburnensis JJ-7(T) (99.6 %), Paenibacillus allorhizosphaerae JJ-447(T) (95.7 %), Flavobacterium buctense T7(T) (97.1 %), and Aquabacterium terrae S2(T) (99.5 %), respectively. Average nucleotide identity and digital DNA-DNA hybridization values between the novel strains and related reference type strains were <95.0 % and <70.0 %, respectively. The major cellular fatty acid in strains W4(T), FW7(T) TW48(T), and UW52(T) was antiso-C-15 : 0. Similarly, strain PT-3(T) revealed iso-C-15 : 0, iso-C-15 : 1 G, iso-C-17 : 0 3-OH, and iso-C-15 : 0 3-OH as its principal fatty acids. On the other hand, RJY3(T) exhibited summed feature 3 (C-16 : 1 omega 7c and/or C-16 : 1 omega 6c), C-16 : 0, summed feature 8 (C-18 : 1 omega 7c and/or C-18 : 1 omega 6c), and C-12 : 0 as its predominant fatty acids. Overall, the polyphasic taxonomic data indicated that strains W4(T), FW7(T), TW48(T), UW52(T), PT-3(T), and RJY3(T) represent novel species within the genera Microbacterium, Paenibacillus, Flavobacterium, and Aquabacterium. Accordingly, we propose the names Microbacterium humicola sp. nov., with the type strain W4(T) (=KCTC 49888(T)=NBRC 116001(T)), Microbacterium terrisoli sp. nov., with the type strain FW7(T) (=KCTC 49859(T)=NBRC 116000(T)), Paenibacillus pedocola sp. nov., with the type strain TW48(T) (=KCTC 43470(T)=NBRC 116017(T)), Paenibacillus silviterrae sp. nov., with the type strain UW52(T) (=KCTC 43477(T)=NBRC 116018(T)), Flavobacterium terrisoli sp. nov., with the type strain PT-3(T) (=KCTC 92106(T)=NBRC 116012(T)), and Aquabacterium humicola sp. nov., with the type strain RJY3(T) (=KCTC 92105(T)=NBRC 115831(T)).