CRISPR screens of tumor-infiltrating NK cells identify genetic checkpoints for CAR-NK therapy

被引：0
|
作者：
Peng, Lei [1 ]
Chen, Sidi [1 ]
机构：
[1] Yale Univ, New Haven, CT 06520 USA
来源：
NATURE BIOTECHNOLOGY | 2024年
关键词：
D O I：
10.1038/s41587-024-02319-8
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We use a CRISPR screening platform based on adeno-associated virus and the Sleeping Beauty transposon (AAV-SB-CRISPR) to perform in vivo CRISPR screens in primary natural killer (NK) cells across four different tumor models, and identify calcium homeostasis modulator family member 2 (CALHM2) as an NK cellular checkpoint protein.
引用
收藏
页数：2
相关论文
共 50 条
  • [21] A structural, genetic and clinical comparison of CAR-T cells and CAR-NK cells: companions or competitors?
    Andrea, Alain E.
    Chiron, Andrada
    Sarrabayrouse, Guillaume
    Bessoles, Stephanie
    Hacein-Bey-Abina, Salima
    FRONTIERS IN IMMUNOLOGY, 2024, 15
  • [22] CAR-NK cells: A promising cellular immunotherapy for cancer
    Xie, Guozhu
    Dong, Han
    Liang, Yong
    Ham, James Dongjoo
    Rizwan, Romee
    Chen, Jianzhu
    EBIOMEDICINE, 2020, 59
  • [23] Study of Affinity Tuning in AMLTargeted CAR-NK Cells
    Rahnama, Ruyan
    Kizerwetter, Monika
    Yang, Huilin
    Christodoulou, Ilias
    Fearnow, Adam
    Vorri, Stamatia
    Spangler, Jamie
    Bonifant, Challice
    CLINICAL LYMPHOMA MYELOMA & LEUKEMIA, 2022, 22 : S257 - S257
  • [24] CAR-NK cells: a promising cellular immunotherapy in lymphoma
    Khanmohammadi, Shaghayegh
    Rezaei, Nima
    EXPERT OPINION ON BIOLOGICAL THERAPY, 2023, 23 (01) : 37 - 47
  • [25] SUPPRESSION OF NK ACTIVITY IN HUMAN TUMOR-INFILTRATING LYMPHOCYTES
    MOY, PM
    GOLUB, SH
    HOLMES, EC
    PROCEEDINGS OF THE AMERICAN ASSOCIATION FOR CANCER RESEARCH, 1983, 24 (MAR): : 231 - 231
  • [26] Generating functional CAR-NK cells for cancer immunotherapy
    Di Gregoli, Karina
    Leonard, Henry
    Rocca, Dan
    de Munnik, Sabrina
    Moiset, Gemma
    Nunan, Robert
    Schewitz-Bowers, Lauren
    Brackenbury, Louise
    CANCER RESEARCH, 2024, 84 (06)
  • [27] GENETIC DELETION OF TIGIT ENHANCES CAR-NK CELL FUNCTION IN THE SOLID TUMOR MICROENVIRONMENT
    Navin, Ishwar
    Dysthe, Matthew
    Baumgartner, Corrine
    Parihar, Robin
    JOURNAL FOR IMMUNOTHERAPY OF CANCER, 2022, 10 : A435 - A435
  • [28] CAR-NK for tumor immunotherapy: Clinical transformation and future prospects
    Wang, Wenxiu
    Jiang, Jingting
    Wu, Changping
    CANCER LETTERS, 2020, 472 : 175 - 180
  • [29] Gene-edited and CAR-NK cells: Opportunities and challenges with engineering of NK cells for immunotherapy
    Wu, Xinyu
    Matosevic, Sandro
    MOLECULAR THERAPY ONCOLYTICS, 2022, 27 : 224 - 238
  • [30] CAR-NK Cells for Cancer Therapy: Molecular Redesign of the Innate Antineoplastic Response
    Cienfuegos-Jimenez, Oscar
    Vazquez-Garza, Eduardo
    Rojas-Martinez, Augusto
    CURRENT GENE THERAPY, 2022, 22 (04) : 303 - 318
12345