CRISPR screens of tumor-infiltrating NK cells identify genetic checkpoints for CAR-NK therapy

被引：0
|
作者：
Peng, Lei [1 ]
Chen, Sidi [1 ]
机构：
[1] Yale Univ, New Haven, CT 06520 USA
来源：
NATURE BIOTECHNOLOGY | 2024年
关键词：
D O I：
10.1038/s41587-024-02319-8
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We use a CRISPR screening platform based on adeno-associated virus and the Sleeping Beauty transposon (AAV-SB-CRISPR) to perform in vivo CRISPR screens in primary natural killer (NK) cells across four different tumor models, and identify calcium homeostasis modulator family member 2 (CALHM2) as an NK cellular checkpoint protein.
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