Isolation and Purification Glycyrrhizic acid and Determination of the Biological Activate Against Microbial Pathogens

Background: The World Health Organization estimates that 80% of the world's population uses plant extracts or their active components in traditional therapies. Recently, there has been a lot of interest in the quest for new antioxidant and antibacterial plant -based medications. Additionally, Glycyrrhiza glabra is a popular food flavoring and sweetening ingredient. Methods: This work was to isolate and purify licorice root's glycyrrhizin acid (GA) and investigate its antimicrobial properties against S. aureus and K. pneumoniae were isolated from urine , blood, wounds, ear water, sewage, soil, and water, totaling 12 isolates of each kind, Analytical method for the measurement of Glycyrrhizic acid from the root extract that has been suitably supported by a mass spectroscopic analysis. Results : The result showed the effects of GA with different concentrations on S. aureus and. K. pneumoniae . Obviously, the MIC range value was in concentration between 18.75 to 37.5 % for S. aureus isolates, and 4.68 to 75 for K. pneumoniae isolates. The result indicated that GA reduced the metabolic activity of cells in biofilm on S. aureus and K. pneumoniae with an inhibition percentage Conclusion : GA may have an effect on cell membrane permeability, biofilm formation, and efflux activity, which inhibits bacterial growth .