Promoter Variation and Gene Expression of mcr-1-Harboring Plasmids in Clinical Isolates of Escherichia coli and Klebsiella pneumoniae from a Chinese Hospital

被引：1
|
作者：
Huang, Bin [1 ]
He, Yuting [1 ]
Ma, Xingyan [1 ]
Cai, Renxin [2 ,3 ]
Zeng, Jianming [2 ,3 ]
Lu, Yang [2 ,3 ]
Zhang, Weizheng [2 ,3 ]
Lan, Kai [2 ,3 ]
E, Sunmei [2 ,3 ]
Tang, Yi-Wei [4 ]
Kreiswirth, Barry N. [5 ]
Chen, Cha [2 ,3 ]
Chen, Liang [5 ]
机构：
[1] Sun Yat Sen Univ, Affiliated Hosp 1, Dept Lab Med, Guangzhou, Guangdong, Peoples R China
[2] Guangzhou Univ Chinese Med, Affiliated Hosp 2, Dept Lab Med, Guangzhou, Guangdong, Peoples R China
[3] Guangdong Prov Hosp Chinese Med, Dept Lab Med, Guangzhou, Guangdong, Peoples R China
[4] Mem Sloan Kettering Canc Ctr, Dept Lab Med, 1275 York Ave, New York, NY 10021 USA
[5] Rutgers State Univ, New Jersey Med Sch, TB Ctr, Publ Hlth Res Inst, Newark, NJ 07102 USA
来源：
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2018年 / 62卷 / 05期
基金：
中国国家自然科学基金;
关键词：
colistin; genomics; plasmids; promoter; gene expression; RESISTANCE;
D O I：
10.1128/AAC.00018-18
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Next-generation sequencing of 6 mcr-1-harboring Escherichia coli and Klebsiella pneumoniae isolates collected from a tertiary care hospital in China revealed significant sequence variations in the regions flanking the mcr-1 gene. While sequence variations significantly affected the expression and promoter activity of mcr-1, the mcr-1 gene expression levels did not correlate with the in vitro colistin resistance levels, which warrants further in-depth investigations.
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页数：5
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