Iterative multi-photon adaptive compensation technique for deep tissue two-photon fluorescence lifetime imaging

被引：0
|
作者：
Wang, Kexin [1 ,2 ,3 ]
Pu, Wenhui [1 ,2 ,3 ]
Qu, Junle [1 ,2 ,3 ]
Liao, Changrui [1 ,2 ,3 ]
Wang, Piping [1 ,2 ,3 ]
He, Jun [1 ,2 ,3 ]
Liu, Liwei [1 ,2 ,3 ]
机构：
[1] Shenzhen Univ, State Key Lab Radio Frequency Heterogeneous Integr, Shenzhen 518060, Peoples R China
[2] Shenzhen Univ, Coll Phys & Optoelect Engn, Key Lab Optoelect Devices & Syst Guangdong Prov, Shenzhen 518060, Peoples R China
[3] Shenzhen Univ, Coll Phys & Optoelect Engn, Minist Educ, Shenzhen 518060, Peoples R China
来源：
CHINESE OPTICS LETTERS | 2024年 / 22卷 / 04期
关键词：
adaptive optics; iterative optimization; two-photon fluorescence lifetime imaging microscopy; wavefront correction; FOCUSING LIGHT; OPTIMIZATION; MICROSCOPY; ALGORITHMS; OPTICS; SENSOR; SYSTEM;
D O I：
10.3788/COL202422.041702
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
Fluorescence lifetime imaging can reveal the high-resolution structure of various biophysical and chemical parameters in a microenvironment quantitatively. However, the depth of imaging is generally limited to hundreds of micrometers due to aberration and light scattering in biological tissues. This paper introduces an iterative multi-photon adaptive compensation technique (IMPACT) into a two-photon fluorescence lifetime microscopy system to successfully overcome aberrations and multiple scattering problems in deep tissues. It shows that 400 correction modes can be achieved within 5 min, which was mainly limited by the frame rate of a spatial light modulator. This system was used for high-resolution imaging of mice brain tissue and live zebrafish, further verifying its superior performance in imaging quality and photon accumulation speed.
引用
收藏
页数：6
相关论文
共 50 条
  • [1] Iterative multi-photon adaptive compensation technique for deep tissue two-photon fluorescence lifetime imaging
    王柯欣
    余文慧
    屈军乐
    廖常锐
    王义平
    何俊
    刘丽炜
    Chinese Optics Letters, 2024, 22 (04) : 94 - 99
  • [2] In vivo fluorescence microscopy via iterative multi-photon adaptive compensation technique
    Kong, Lingjie
    Cui, Meng
    OPTICS EXPRESS, 2014, 22 (20): : 23786 - 23794
  • [3] In vivo neuroimaging through the highly scattering tissue via iterative multi-photon adaptive compensation technique
    Kong, Lingjie
    Cui, Meng
    OPTICS EXPRESS, 2015, 23 (05): : 6145 - 6150
  • [4] Two-photon excitation in fluorescence lifetime imaging
    Gerritsen, HC
    Vroom, J
    Sytsma, J
    FLUORESCENCE MICROSCOPY AND FLUORESCENT PROBES, VOL 2, 1998, : 55 - 62
  • [5] Applications of two-photon fluorescence microscopy in deep tissue imaging
    Dong, CY
    Yu, B
    Hsu, L
    Kaplan, P
    Blankschstein, D
    Langer, R
    So, PTC
    OPTICAL SENSING, IMAGING, AND MANIPULATION FOR BIOLOGICAL AND BIOMEDICAL APPLICATIONS, 2000, 4082 : 105 - 114
  • [6] Imaging theory of two-photon and multi-photon confocal scanning microscopy
    Tang, Zhilie
    Liang, Ruisheng
    Chang, Hongsen
    Wuli Xuebao/Acta Physica Sinica, 2000, 49 (06): : 1076 - 1080
  • [7] The imaging theory of two-photon and multi-photon confocal scanning microscopy
    Tang, ZL
    Liang, RS
    Chang, HS
    ACTA PHYSICA SINICA, 2000, 49 (06) : 1076 - 1080
  • [8] The imaging theory of two-photon and multi-photon confocal scanning microscopy
    Tang, Zhi-Lie
    Liang, Rui-Sheng
    Chang, Hong-Sen
    2000, Science Press (49):
  • [9] Combining two-photon excitation with fluorescence lifetime imaging
    Gerritsen, HC
    Vroom, JM
    de Grauw, CJ
    IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE, 1999, 18 (05): : 31 - 36
  • [10] Deep-tissue multi-photon fluorescence lifetime microscopy for intravital imaging of protein-protein interactions
    Fruhwirth, G. O.
    Matthews, D. R.
    Brock, A.
    Keppler, M.
    Vojnovic, B.
    Ng, T.
    Ameer-Beg, S.
    MULTIPHOTON MICROSCOPY IN THE BIOMEDICAL SCIENCES IX, 2009, 7183
12345