Parvalbumin Gene: A Valuable Marker for Pike Authentication and Allergen Risk Assessment

被引:0
|
作者
Cermakova, Eliska [1 ,2 ]
Mukherjee, Subham [1 ,3 ,4 ]
Novakova, Denisa [2 ]
Horka, Petra [4 ]
Zdenkova, Kamila [2 ]
Demnerova, Katerina [2 ]
机构
[1] Food Res Inst Prague, Dept Chem Biochem & Food Microbiol, Prague 10, Czech Republic
[2] Univ Chem & Technol, Dept Biochem & Microbiol, Prague 6, Czech Republic
[3] Keele Univ, Lennard Jones Sch Chem & Phys Sci, Keele ST5 5BG, Staffs, England
[4] Charles Univ Prague, Inst Environm Studies, Fac Sci, Prague 2, Czech Republic
关键词
food allergy; DNA; Esox; food fraud; PCR; LAMP; MEDIATED ISOTHERMAL AMPLIFICATION; ESOX-SPP; ACTINOPTERYGII; RAPID DETECTION; NORTHERN PIKE; MITOCHONDRIAL GENOME; DNA EXTRACTION; 2ND INTRON; FISH; LUCIUS; PCR;
D O I
10.1021/acs.jafc.4c01410
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Fish from the pike (Esox) genus are valued in gastronomy for their superior meat quality. However, they can cause allergic reactions in sensitive consumers. This work aimed to fill the gap in the detection of pike allergens using molecular-biological techniques. New, fast, and accurate loop-mediated isothermal amplification (LAMP) and real-time PCR (qPCR) assays were designed to detect pike DNA using the parvalbumin gene as a marker. LAMP was assessed by electrophoresis, SYBR green optical detection, and real-time fluorescence detection. The latter was the most sensitive, detecting as little as 0.78 ng of pike DNA; the qPCR detection limit was 0.1 ng. The LAMP analysis took 20-70 min, which is significantly faster than qPCR. The study provides reliable detection and quantification of the parvalbumin gene in both fresh and processed samples and further highlights the versatility of the use of the parvalbumin gene for the authentication of food products and consumer protection via refined allergen risk assessment that is independent of the type of tissue or food processing method used.
引用
收藏
页码:12788 / 12797
页数:10
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