Structure and function of rhizosphere soil microbial communities associated with root rot of Knoxia roxburghii

被引:0
|
作者
Liu, Chunju [1 ,2 ]
Li, Heng [3 ]
Dong, Jiahong [1 ]
He, Xiahong [4 ]
Zhang, Lei [1 ]
Qiu, Bin [1 ]
机构
[1] Yunnan Univ Chinese Med, Sch Chinese Mat Med, Kunming, Yunnan, Peoples R China
[2] Yunnan Agr Univ, Coll Plant Protect, Kunming, Yunnan, Peoples R China
[3] R&D Ctr Yunnan Yuntianhua Co Ltd, Kunming, Yunnan, Peoples R China
[4] Southwest Forestry Univ, Sch Landscape Architecture & Hort Sci, Kunming, Yunnan, Peoples R China
关键词
Knoxia roxburghii; root rot; rhizosphere microorganism; physicochemical properties; enzyme activities; PANAX-NOTOGINSENG; CAUSAL AGENT; DIVERSITY;
D O I
10.3389/fmicb.2024.1424633
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The microbial communities in rhizosphere soil play important roles in plant health and crop productivity. However, the microbial community structure of rhizosphere soil still remains unclear. In this study, the composition, diversity and function of the microbial communities in the rhizosphere soil of healthy and diseased plants were compared using Illumina MiSeq high-throughput sequencing. The Sobs (richness) and Shannon (diversity) indices of the soil microbial communities were higher in the rhizospheres of 2- and 3-year-old susceptible plants than in those of the healthy plants. With the increase in planting time, the numbers of fungi tended to decrease, while those of the bacteria tended to increase. Fungal diversity could be used as a biological indicator to measure the health of Knoxia roxburghii. The microbial composition and differential analyses revealed that the rhizosphere soil infested with fungi had a higher relative abundance at the phylum level in Ascomycota and Basidiomycota, while the bacteria had a higher relative abundance of Chloroflexi and a lower relative abundance of Actinobacteriota. At the genus level, the rhizosphere soil infested with fungi had relatively more abundant unclassified_f__Didymellaceae and Solicoccozyma and relatively less abundant Saitozyma and Penicillium. The bacterial genus norank_f__Gemmatimonadaceae was the most abundant, while Arthrobacter was less abundant. In addition, the abundance of Fusarium in the fungal community varied (p = 0.001). It tended to increase in parallel with the planting years. Therefore, it was hypothesized that the change in the community composition of Fusarium may be the primary reason for the occurrence of root rot in K. roxburghii, and the change in the abundance of Fusarium OTU1450 may be an indication of the occurrence of root rot in this species. The community function and prediction analyses showed that the pathogenic fungi increased with the increase in planting years. In general, soil fungi can be roughly divided into three types, including pathotrophs, symbiotrophs, and saprotrophs. An analysis of the differences in the prediction of different rhizosphere functions showed that D and L were significantly different in the COG enrichment pathway of the K. roxburghii rhizosphere bacteria (p < 0.05). The soil physical and chemical properties, including the pH, AK, total potassium (TK), and catalase (S_CAT), had the most significant effect on the soil fungal community, and most of the soil physical and chemical properties significantly correlated with the bacterial community. This study demonstrated that the occurrence of root rot had an important effect on the diversity, structure and composition of microbial communities. In addition, the results will provide a theoretical basis to prevent and control root rot in K. roxburghii.
引用
下载
收藏
页数:13
相关论文
共 50 条
  • [21] Unraveling the characteristics of the microbial community and potential pathogens in the rhizosphere soil of Rehmannia glutinosa with root rot disease
    Wang, Ruifei
    Wang, Yan
    Yang, Qingxiang
    Kang, Chunxiao
    Li, Mingjun
    APPLIED SOIL ECOLOGY, 2018, 130 : 271 - 279
  • [22] The Influence of the Genotype and Planting Density on the Structure and Composition of Root and Rhizosphere Microbial Communities in Maize
    Zhang, Yang
    Lin, Jianxin
    Chen, Shanhu
    Lu, Heding
    Liao, Changjian
    MICROORGANISMS, 2023, 11 (10)
  • [23] Alternate micro-sprinkler irrigation and organic fertilization decreases root rot and promotes root growth of Panax notoginseng by improving soil environment and microbial structure in rhizosphere soil
    Zang, Zhennan
    Yang, Qiliang
    Liang, Jiaping
    Yang, Ying
    Li, Na
    Wang, Haidong
    Guo, Jinjin
    Yang, Ling
    INDUSTRIAL CROPS AND PRODUCTS, 2023, 202
  • [24] Diverse crop rotations influence the bacterial and fungal communities in root, rhizosphere and soil and impact soil microbial processes
    Town, Jennifer R.
    Gregorich, Edward G.
    Drury, Craig F.
    Lemke, Reynald
    Phillips, Lori A.
    Helgason, Bobbi L.
    APPLIED SOIL ECOLOGY, 2022, 169
  • [25] Divergent rhizosphere and non-rhizosphere soil microbial structure and function in long-term warmed steppe due to altered root exudation
    Yu, Yang
    Zhou, Yong
    Janssens, Ivan A.
    Deng, Ye
    He, Xiaojia
    Liu, Lingli
    Yi, Yin
    Xiao, Nengwen
    Wang, Xiaodong
    Li, Chao
    Xiao, Chunwang
    GLOBAL CHANGE BIOLOGY, 2024, 30 (01)
  • [26] The influence of rhizosphere soil fungal diversity and complex community structure on wheat root rot disease
    Zhang, Xuejiang
    Wang, Heyun
    Que, Yawei
    Yu, Dazhao
    Wang, Hua
    PEERJ, 2021, 9
  • [27] Functional Potential of Soil Microbial Communities in the Maize Rhizosphere
    Li, Xiangzhen
    Rui, Junpeng
    Xiong, Jingbo
    Li, Jiabao
    He, Zhili
    Zhou, Jizhong
    Yannarell, Anthony C.
    Mackie, Roderick I.
    PLOS ONE, 2014, 9 (11):
  • [28] Effect of Huanglongbing on the structure and functional diversity of microbial communities associated with citrus rhizosphere
    Trivedi, P.
    He, Z.
    Van Nostrand, J. D.
    Zhou, J.
    Albrigo, G.
    Wang, N.
    PHYTOPATHOLOGY, 2011, 101 (06) : S178 - S178
  • [29] Huanglongbing alters the structure and functional diversity of microbial communities associated with citrus rhizosphere
    Trivedi, Pankaj
    He, Zhili
    Van Nostrand, Joy D.
    Albrigo, Gene
    Zhou, Jizhong
    Wang, Nian
    ISME JOURNAL, 2012, 6 (02): : 363 - 383
  • [30] Huanglongbing alters the structure and functional diversity of microbial communities associated with citrus rhizosphere
    Pankaj Trivedi
    Zhili He
    Joy D Van Nostrand
    Gene Albrigo
    Jizhong Zhou
    Nian Wang
    The ISME Journal, 2012, 6 : 363 - 383