Performance characteristics of genus or species-specific Polymerase Chain Reaction (PCR) for the microbial diagnosis of joint infections: A systematic review and meta-analysis

被引:0
|
作者
Subedi, Shradha [1 ,2 ,3 ,8 ]
Isler, Burcu [1 ,4 ]
Ezure, Yukiko [1 ,5 ]
Furuya-Kanamori, Luis [1 ]
Eriksson, Lars [6 ]
Paterson, David L. [1 ,7 ]
Harris, Patrick N. A. [1 ,3 ]
机构
[1] Univ Queensland, Fac Med, Ctr Clin Res, Herston, Qld, Australia
[2] Sunshine Coast Hosp & Hlth Serv, Britinya, Qld, Australia
[3] Pathol Queensland, Microbiol Lab, Brisbane, Qld, Australia
[4] Princess Alexandra Hosp, Infect Management Serv, Brisbane, Qld, Australia
[5] Univ Queensland, Sch Publ Hlth, Brisbane, Australia
[6] Univ Queensland, Herston Hlth Sci Lib, Brisbane, Qld, Australia
[7] ADVANCE ID, Singapore, Singapore
[8] Sunshine Coast Univ Hosp, 6 Doherty St, Birtinya, Qld 4575, Australia
关键词
Prosthetic joint infections; Polymerase chain reaction; Diagnostic accuracy; Septic arthritis; MULTIPLEX-PCR; SYNOVIAL-FLUID; TEST ACCURACY; SONICATION FLUID; HIP; ASPIRATION; CULTURE; ARTHRITIS; STILL;
D O I
10.1016/j.diagmicrobio.2024.116422
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Joint infections cause significant morbidity and mortality. Rapid diagnosis enables prompt initiation of appropriate antimicrobial therapy and surgical treatment. We conducted a systematic review and meta-analysis to evaluate the accuracy of genus- or species-specific polymerase chain reaction (PCR) in diagnosing joint infections. The literature databases were searched for articles from January 2010 to December 2022. The meta- analysis using the split component synthesis (SCS) method, included 20 studies with 2,457 adult participants. The pooled sensitivity, specificity, diagnostic odds ratio, and AUC of PCR were 49 % (95 % CI [37.9-60.2]), 95.7 % (95 % CI [91.6-97.8]), 21.32, and 0.82 respectively. Sensitivity was highest for sonicate fluid and lowest for periprosthetic tissue. The mean turnaround time to results was 4.7 hours (SD 1.1). PCR is a favourable option for diagnosing joint infections due to its rapid results, but it has low sensitivity. To enhance diagnostic yield, the test should be used in conjunction with other methods.
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页数:8
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