Development of Methyl 2-aminobenzoate Reference Material in a Biocidal Product Matrix

The utilization of methyl 2-aminobenzoate as a biocide and pesticide has raised concerns regarding its potential toxicity. To assess its safety, it is crucial to determine its quantity and related toxicity using reference materials (RMs) or certified reference materials (CRMs). As an RM and CRM containing methyl 2-aminobenzoate within a biocidal product matrix is currently unavailable, this study aimed to produce a high-quality RM containing methyl 2-aminobenzoate, ensuring its homogeneity and stability, following the ISO Guide 35 and ISO 17034. The study determined that the produced RM exhibited homogeneity, as indicated by a calculated F-value (1.91) smaller than the critical F-value (3.02). In the assessment of isochronous short-term stability, the slope of the linear regression for the RM showed no statistically significant difference from zero when stored at temperatures of 4, 18, and 60 degrees C for 4 weeks. Regarding classical long-term stability, the RM demonstrated sustained stability over the course of one year when stored at 4 degrees C. This study has successfully developed an RM for monitoring methyl 2-aminobenzoate in biocides and pesticides. Its quality underwent rigorous evaluation, confirming both homogeneity and stability.