Curing of a field strain of Salmonella enterica serovar Infantis isolated from poultry from its highly stable pESI like plasmid

被引:1
|
作者
Gruzdev, Nadya [1 ]
Katz, Chen [1 ]
Yadid, Itamar [1 ,2 ]
机构
[1] Migal Galilee Res Inst, IL-1101602 Kiryat Shmona, Israel
[2] Tel Hai Coll, IL-1220800 Upper Galilee, Israel
关键词
Salmonella enterica serovar Infantis; Megaplasmid-pESI; Plasmid curing; Toxin-antitoxin addiction systems; RESISTANT SALMONELLA; VIRULENCE;
D O I
10.1016/j.mimet.2024.106959
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Salmonella enterica serovar Infantis (S. infantis) is an important emerging pathogen, associated with poultry and poultry products and related to an increasing number of human infections in many countries. A concerning trend among S. infantis isolates is the presence of plasmid-mediated multidrug resistance. In many instances, the genes responsible for this resistance are carried on a megaplasmid known as the plasmid of emerging S. infantis (pESI) or pESI like plasmids. Plasmids can be remarkably stable due to the presence of multiple replicons and postsegregational killing systems (PSKs), which contribute to their maintenance within bacterial populations. To enhance our understanding of S. infantis and its multidrug resistance determinants toward the development of new vaccination strategies, we have devised a new method for targeted plasmid curing. This approach effectively overcomes plasmid addiction by leveraging the temporal overproduction of specific antitoxins coupled with the deletion of the partition region. By employing this strategy, we successfully generated a plasmid-free strain from a field isolate derived from S. infantis 119,944. This method provides valuable tools for studying S. infantis and its plasmid-borne multidrug resistance mechanisms and can be easily adopted for plasmid curing from other related bacteria.
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页数:6
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