Construction of a coumarin-based fluorescent probe for accurately visualizing hydrogen sulfide in live cells and zebrafish

Hydrogen sulfide (H2S), an important gas signaling molecule, is a regulator of many physiological processes, and its abnormal levels are closely related to the onset and progression of disease. It is vital to develop methods for specific tracking of H2S in clinical diagnosis and treatment. In this study, we designed an ultrasensitive and highly stable coumarin-based fluorescent probe Cou-H2S. Through the H2S-initiated tandem reaction, Cou-H2S successfully achieved highly selective and super-fast detection of H2S. Cou-H2S was successfully applied to the monitoring of endogenous and exogenous H2S at the cellular level and verified the validity of the detection of H2S in the LPS-induced zebrafish model. Therefore, Cou-H2S might provide new insights into the study of H2S-related diseases. Hydrogen sulfide (H2S), an important gas signaling molecule, is a regulator of many physiological processes, and its abnormal levels are closely related to the onset and progression of disease.