Reprogramming of DNA methylation and changes of gene expression in grafted Hevea brasiliensis

被引：0
|
作者：
Li, Hui-Liang [1 ,2 ,3 ,4 ,5 ]
Wang, Ying [1 ,2 ]
Guo, Dong [1 ,2 ,3 ,4 ]
Zhu, Jia-Hong [1 ,2 ,3 ,4 ]
Wang, Yu [1 ,2 ,3 ,4 ,5 ]
Dai, Hao-Fu [1 ,2 ]
Peng, Shi-Qing [1 ,2 ]
机构：
[1] Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Haikou, Hainan, Peoples R China
[2] Hainan Acad Trop Agr Resource, Key Lab Biol & Genet Resources Hainan Prov, Haikou, Hainan, Peoples R China
[3] Chinese Acad Trop Agr Sci, Natl Key Lab Trop Crop Breeding, Sanya, Hainan, Peoples R China
[4] Chinese Acad Trop Agr Sci, Sanya Res Inst, Sanya, Hainan, Peoples R China
[5] Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Hainan Key Lab Biosafety Monitoring & Mol Breeding, Haikou, Hainan, Peoples R China
来源：
FRONTIERS IN PLANT SCIENCE | 2024年 / 15卷
基金：
中国国家自然科学基金; 海南省自然科学基金;
关键词：
Hevea brasiliensis; grafting; whole genome bisulfite sequencing; DNA methylation; gene expression; GENOME; IDENTIFICATION; PLANTS; HISAT;
D O I：
10.3389/fpls.2024.1407700
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Rubber tree (Hevea brasiliensis) is reproduced by bud grafting for commercial planting, but significant intraclonal variations exist in bud-grafted clones. DNA methylation changes related to grafting may be partly responsible for intraclonal variations. In the current study, whole-genome DNA methylation profiles of grafted rubber tree plants (GPs) and their donor plants (DPs) were evaluated by whole-genome bisulfite sequencing. Data showed that DNA methylation was downregulated and DNA methylations in CG, CHG, and CHH sequences were reprogrammed in GPs, suggesting that grafting induced the reprogramming of DNA methylation. A total of 5,939 differentially methylated genes (DMGs) were identified by comparing fractional methylation levels between GPs and DPs. Transcriptional analysis revealed that there were 9,798 differentially expressed genes (DEGs) in the DP and GP comparison. A total of 1,698 overlapping genes between DEGs and DMGs were identified. These overlapping genes were markedly enriched in the metabolic pathway and biosynthesis of secondary metabolites by Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Global DNA methylation and transcriptional analyses revealed that reprogramming of DNA methylation is correlated with gene expression in grafted rubber trees. The study provides a whole-genome methylome of rubber trees and an insight into the molecular mechanisms underlying the intraclonal variations existing in the commercial planting of grafted rubber trees.
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页数：13
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