Ohwia caudata aqueous extract attenuates senescence in aging adipose-derived mesenchymal stem cells

被引：0
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作者：
Ho, Tsung-Jung [1 ,2 ,3 ]
Tsai, Bruce Chi-Kang [4 ]
Debakshee, Goswami [4 ]
Shibu, Marthandam Asokan [4 ,5 ]
Kuo, Chia-Hua [6 ,7 ,8 ,9 ]
Lin, Chih-Hsueh [10 ,11 ]
Lin, Pi-Yu [12 ]
Lin, Shinn-Zong [13 ,14 ]
Kuo, Wei-Wen [15 ,16 ,17 ]
Huang, Chih-Yang [4 ,18 ,19 ,20 ,21 ,22 ]
机构：
[1] Hualien Tzu Chi Hosp, Buddhist Tzu Chi Med Fdn, Dept Chinese Med, Hualien, Taiwan
[2] Hualien Tzu Chi Hosp, Buddhist Tzu Chi Med Fdn, Integrat Ctr Tradit Chinese & Modern Med, Hualien, Taiwan
[3] Tzu Chi Univ, Sch Postbaccalaureate Chinese Med, Coll Med, Hualien, Taiwan
[4] Hualien Tzu Chi Hosp, Buddhist Tzu Chi Med Fdn, Cardiovasc & Mitochondrial Related Dis Res Ctr, Hualien, Taiwan
[5] Bharathiar Univ, Dept Biotechnol, Coimbatore, Tamil Nadu, India
[6] Univ Taipei, Inst Sports Sci, Taipei, Taiwan
[7] Univ Taipei, Lab Exercise Biochem, Tianmu Campus, Taipei, Taiwan
[8] Coll William & Mary, Dept Kinesiol & Hlth Sci, Williamsburg, VA USA
[9] Soochow Univ, Sch Phys Educ & Sports Sci, Suzhou, Peoples R China
[10] China Med Univ, Coll Med, Sch Med, Taichung, Taiwan
[11] China Med Univ Hosp, Dept Family Med, Taichung, Taiwan
[12] Buddhist Compass Relief Tzu Chi Fdn, Hualien, Taiwan
[13] Buddhist Tzu Chi Med Fdn, Bioinnovat Ctr, Hualien, Taiwan
[14] Hualien Tzu Chi Hosp, Buddhist Tzu Chi Med Fdn, Dept Neurosurg, Hualien, Taiwan
[15] China Med Univ, Coll Life Sci, Dept Biol Sci & Technol, Taichung, Taiwan
[16] China Med Univ, PhD Program Biotechnol Ind, Taichung, Taiwan
[17] China Med Univ, Sch Pharm, Taichung, Taiwan
[18] China Med Univ, Grad Inst Biomed Sci, Taichung, Taiwan
[19] China Med Univ, China Med Univ Hosp, Dept Med Res, Taichung, Taiwan
[20] Asia Univ, Dept Med Lab Sci & Biotechnol, Taichung, Taiwan
[21] Tzu Chi Univ Sci & Technol, Buddhist Tzu Chi Med Fdn, Ctr Gen Educ, Hualien, Taiwan
[22] Tzu Chi Univ Sci & Technol, Hualien Tzu Chi Hosp, Buddhist Tzu Chi Med Fdn, Cardiovasc & Mitochondrial Related Dis Res Ctr, Hualien, Taiwan
来源：
HELIYON | 2024年 / 10卷 / 09期
关键词：
Adipose -derived mesenchymal stem cells; Pluripotency; Self; -renewal; Herbal medicine; Ohwia caudata; Desmodium caudatum; DNA-DAMAGE; DOXORUBICIN; MECHANISMS; KLOTHO; CANCER; MODEL;
D O I：
10.1016/j.heliyon.2024.e29729
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Stem cells exhibit pluripotency and self -renewal abilities. Adipose -derived mesenchymal stem cells can potentially be used to reconstruct various tissues. They possess significant versatility and alleviate various aging -related diseases. Unfortunately, aging leads to senescence, apoptosis, and a decline in regenerative capacity in adipose -derived mesenchymal stem cells. These changes necessitate a strategy to mitigate the effects of aging on stem cells. Ohwia caudata ( O. caudata ) has therapeutic effects against several illnesses. However, studies on whether O. caudata has therapeutic effects against aging are lacking. In this study, we aimed to identify potential therapeutic anti -aging effects in the crude aqueous extract of O. caudata on adipose -derived mesenchymal stem cells. Using 0.1 mu M doxorubicin, we induced aging in human adipose -derived mesenchymal stem cells (hADMSCs) and evaluated whether various concentrations of O. caudata aqueous extract exhibit anti -aging effects on them. The O. caudata extract exhibited significant antioxidant effects on hADMSCs without any toxicity. Furthermore, after treatment with the O. caudata aqueous extract, the levels of mitochondrial superoxide, DNA double -strand breaks, and telomere shortening were reduced in the hADMSCs subjected to doxorubicin-induced aging. The extract also suppressed doxorubicin-induced aging by upregulating klotho and downregulating p21 in hADMSCs. These findings indicated that the O. caudata extract exhibited anti -aging properties that modulated hADMSC homeostasis. Therefore, it could be a potential candidate for restoring the self -renewal ability and multipotency of aging hADMSCs.
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页数：11
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