Qualitative and Quantitative Analysis of Sea Buckthorn Seed Extract and Its Antioxidant Effect in Liver of Aging Mice

This research studied the qualitative analysis of sea buckthorn seed extract (SBSE), prepared from sea buckthorn seed residue by ethanol extraction and macroporous resin purification, by ultra-performance liquid chromatography coupled with quadruple time-of-flight tandem mass spectrometry (UPLC-QTOF-MS/MS). The bioactive components of SBSE were quantitatively determined by ultra performance liquid chromatography coupled with triple quadruple mass spectrometry(UPLC-QQQ-MS). Meanwhile, the antioxidant effect of SBSE in D-galactose-induced aging mice was studied. The mice were administrated by gavage with SBSE at different dosages for 42 days. Total antioxidant capacity (T-AOC), the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) and the contents of malondialdehyde(MDA) and lipofuscin (LP) in liver tissue were measured. The results obtained were as follows: A total of 24 compounds were identified by UPLC-QTOF-MS/MS, 14 compounds of which were characterized by comparing with reference substances and quantitatively determined by UPLC-QQQ-MS in the negative ion multiple reaction monitoring (MRM)mode. Kaempferol and its derivatives were the most abundant constituents in SBSE ((667.94 ± 5.61) μg/mL), quercetin and its derivatives were in the middle ((204.01 ± 0.04) μg/mL), and procyanidins were the least abundant ((33.97 ± 0.49) μg/mL).The administration of SBSE to aging mice could increase the activities of GSH-Px, SOD and CAT in liver tissue. Compared with the D-galactose-induced model group, hepatic T-AOC activity in the low-dosage group was significantly increased(P © 2019, China Food Publishing Company. All right reserved.