Rapid detection of West Nile and Dengue viruses from mosquito saliva by loop-mediated isothermal amplification and displaced probes

被引:0
|
作者
Kim, Dongmin [1 ]
DeBriere, Terry J. [2 ]
Eastmond, Bradley H. [1 ]
Alomar, Abdullah A. [1 ]
Yaren, Ozlem [3 ,4 ]
McCarter, Jacquelyn [3 ,4 ]
Bradley, Kevin M. [4 ]
Benner, Steven A. [3 ,4 ]
Alto, Barry W. [1 ]
Burkett-Cadena, Nathan D. [1 ]
机构
[1] Univ Florida, Florida Med Entomol Lab, Vero Beach, FL 32962 USA
[2] TrakitNow Inc, Columbia, SC USA
[3] Firebird Biomol Sci LLC, Alachua, FL 32615 USA
[4] Fdn Appl Mol Evolut, Alachua, FL 32615 USA
来源
PLOS ONE | 2024年 / 19卷 / 02期
关键词
EQUINE ENCEPHALITIS-VIRUS; ARBOVIRUS SURVEILLANCE; VECTOR COMPETENCE; AEDES-AEGYPTI; DIPTERA; LAMP; CULICIDAE; SUSCEPTIBILITY; IDENTIFICATION; TEMPERATURE;
D O I
10.1371/journal.pone.0298805
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Arthropod-borne viruses are major causes of human and animal disease, especially in endemic low- and middle-income countries. Mosquito-borne pathogen surveillance is essential for risk assessment and vector control responses. Sentinel chicken serosurveillance (antibody testing) and mosquito pool screening (by RT-qPCR or virus isolation) are currently used to monitor arbovirus transmission, however substantial time lags of seroconversion and/or laborious mosquito identification and RNA extraction steps sacrifice their early warning value. As a consequence, timely vector control responses are compromised. Here, we report on development of a rapid arbovirus detection system whereby adding sucrose to reagents of loop-mediated isothermal amplification with displaced probes (DP-LAMP) elicits infectious mosquitoes to feed directly upon the reagent mix and expectorate viruses into the reagents during feeding. We demonstrate that RNA from pathogenic arboviruses (West Nile and Dengue viruses) transmitted in the infectious mosquito saliva was detectable rapidly (within 45 minutes) without RNA extraction. Sucrose stabilized viral RNA at field temperatures for at least 48 hours, important for transition of this system to practical use. After thermal treatment, the DP-LAMP could be reliably visualized by a simple optical image sensor to distinguish between positive and negative samples based on fluorescence intensity. Field application of this technology could fundamentally change conventional arbovirus surveillance methods by eliminating laborious RNA extraction steps, permitting arbovirus monitoring from additional sites, and substantially reducing time needed to detect circulating pathogens.
引用
收藏
页数:15
相关论文
共 50 条
  • [1] Loop-mediated isothermal amplification technology for rapid detection of plant viruses
    James, D.
    CANADIAN JOURNAL OF PLANT PATHOLOGY-REVUE CANADIENNE DE PHYTOPATHOLOGIE, 2009, 31 (01): : 115 - 115
  • [2] Rapid discriminative detection of dengue viruses via loop mediated isothermal amplification
    Kim, Jong-Gil
    Baek, Seung Hoon
    Kim, Seungrok
    Kim, Hae In
    Lee, Seung Woo
    Le Minh Tu Phan
    Kailasa, Suresh Kumar
    Park, Tae Jung
    TALANTA, 2018, 190 : 391 - 396
  • [3] Rapid detection of tobacco viruses by reverse transcription loop-mediated isothermal amplification
    Lei Zhao
    Julong Cheng
    Xingan Hao
    Xiaoman Tian
    Yunfeng Wu
    Archives of Virology, 2012, 157 : 2291 - 2298
  • [4] Rapid detection of tobacco viruses by reverse transcription loop-mediated isothermal amplification
    Zhao, Lei
    Cheng, Julong
    Hao, Xingan
    Tian, Xiaoman
    Wu, Yunfeng
    ARCHIVES OF VIROLOGY, 2012, 157 (12) : 2291 - 2298
  • [5] Rapid Detection of Watermelon Viruses by Reverse Transcription Loop-Mediated Isothermal Amplification
    Zhao, Lei
    Liu, Yang
    Wu, Yunfeng
    Hao, Xingan
    JOURNAL OF PHYTOPATHOLOGY, 2016, 164 (05) : 330 - 336
  • [6] Real-time reverse transcription loop-mediated isothermal amplification for rapid detection of West Nile virus
    Parida, M
    Posadas, G
    Inoue, S
    Hasebe, F
    Morita, K
    JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (01) : 257 - 263
  • [7] Loop-mediated isothermal amplification for the rapid detection of Salmonella
    Hara-Kudo, Y
    Yoshino, M
    Kojima, T
    Ikedo, M
    FEMS MICROBIOLOGY LETTERS, 2005, 253 (01) : 155 - 161
  • [8] Detection of human influenza A viruses by loop-mediated isothermal amplification
    Poon, LLM
    Leung, CSW
    Chan, KH
    Lee, JHC
    Yuen, KY
    Guan, Y
    Peiris, JSA
    JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (01) : 427 - 430
  • [9] Rapid detection of Anguina agrostis by loop-mediated isothermal amplification
    Yu, Lu-zhen
    Song, Shao-yi
    Yu, Cui
    Jiao, Bin-bin
    Tian, Yi-min
    Li, You-jun
    EUROPEAN JOURNAL OF PLANT PATHOLOGY, 2020, 156 (03) : 819 - 825
  • [10] Rapid detection of Mycoplasma synoviae by loop-mediated isothermal amplification
    Olimpia Kursa
    Grzegorz Woźniakowski
    Grzegorz Tomczyk
    Anna Sawicka
    Zenon Minta
    Archives of Microbiology, 2015, 197 : 319 - 325