Biochemical Characterization of Endophytic Rhizobacteria with Biocontrol Activity Against Phytophthora palmivora and Lasiodiplodia theobromae

被引:0
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作者
Fabricio Canchignia-Martinez, Hayron [1 ]
Fernando Vera-Benites, Luis [1 ]
Nicolle Tapia-Quintana, Dayanara [1 ]
Virgilio Cedeno-Moreira, Angel [2 ]
Garcia-Intriago, Erick [3 ]
John Macias-Holguin, Cristhian [3 ]
机构
[1] Univ Tecn Estatal Quevedo, Lab Microbiol Mol, Dept Biotecnol, Fac Ciencias Agrarias & Forest, Ave Quito Km 1-5 Via Santo Domingo Tsachila, Quevedo 120501, Ecuador
[2] Univ Tecn Estatal Quevedo, Lab Biol & Microbiol, Campus Expt Maria, Quevedo, Ecuador
[3] Univ Tecn Estatal Quevedo, Lab Quim & Bioquim, Via Quevedo El Empalme Km 7, Quevedo 120501, Ecuador
关键词
proteolytic activity; antagonism; lytic enzymes; cell extracts; siderophores; BLACK POD; PSEUDOMONAS-FLUORESCENS; SOLUBILIZING BACTERIA; SYSTEMIC RESISTANCE; CROWN ROT; CACAO L; GROWTH; RHIZOSPHERE; ANTAGONIST; MANAGEMENT;
D O I
10.28940/terra.v42i0.1807
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
The use of rhizobacteria has the ability to colonize the root system, promote plant growth and biocontrol or suppress phytopathogenic fungi. The objective of this research was to select and analyze fluorescent rhizobacteria solubilizing nitrogen, phosphorus and potassium, producing lytic enzymes with antagonistic activity towards P. palmivora and L. theobromae. The bacteria were isolated from root and leaf tissue of endemic CCN-51 cacao cultivars from the provinces of Los Rios, Santa Elena and Esmeralda. Characterization of antagonistic activity was based on the selection of proteolytic activity, siderophore production and fluorescent pigmentation under ultra violet light. In addition, antagonistic activity was performed using cell extracts. Nine bacterial strains (BF 567, FZ 9-7, LH 5-10, MH 4-20, MN 5-20, MN 5-19, AC3, PV-25) with protease production, siderophore production and fluorescence emission were selected. The ability of the bacteria to solubilize complex minerals (nitrogen, phosphorus and potassium) was analyzed and their solubilization efficiency varied, ranging from 50 to 100 %. The antagonistic factor of cell supernatants from FZ 9-7, BF 567 and PV-25 inhibited mycelial growth toward P. palmivora and L. theobromae by 40, 60 and 85%, respectively. The use of rhizobacteria could be used as an alternative biofertilizer to chemical fertilizers and biological control of phytopathogenic soil fungi through their volatile emissions.
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页数:10
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