Recent Progress in Small-molecule Fuorescent Probes for in situ Imaging and Detection of Enzyme

被引：0

作者：

Zhang L. ^{[1
]}

Huang Z. ^{[1
]}

Shen Q. ^{[1
]}

Ju L. ^{[2
]}

Wu Q. ^{[1
]}

Yu C. ^{[1
]}

机构：

[1] School of Flexible Electronics（Future Technologies）, Nanjing Tech University, Nanjing

[2] The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing

来源：

Faguang Xuebao/Chinese Journal of Luminescence | 2023年 / 44卷 / 11期

关键词：

enzyme; in situ imaging; recent progress; small-molecule fluorescent probes;

D O I：

10.37188/CJL.20230128

中图分类号：

学科分类号：

摘要：

Enzyme widely exists in various life activities. Some diseases are closely related to abnormal expression of active enzymes. Accurate detection of enzyme expression levels and in situ imaging provide a powerful basis for diagnosis and treatment of related diseases. Up to now，a large number of detection technologies have been developed，among which the fluorescent technology represented by small-molecule fluorescent probes has advantages such as non-invasive，high sensitivity，low detection limit，fast response time and good biocompatibility. It is favored in the detection of biological enzyme. However，as small-molecule fluorescent probes are used for detection，they tend to diffuse at the active sites of enzyme，resulting in poor spatial and temporal resolution of the probes. Therefore，in order to improve the spatial and temporal resolution of imaging detection and reduce background interference and false positives，the design of in situ imaging has been proposed，which has become one of the research focuses of optical imaging. At present，researchers have reported that a variety of small-molecular fluorescent probes have been used in the design of enzyme in situ imaging and achieved remarkable results. This review will introduce the design strategy of small-molecular fluorescent probes for enzyme detection and the research progress in situ imaging，hoping to provide some inspirations for researchers in this field. © 2023 Chines Academy of Sciences. All rights reserved.

引用

页码：2057 / 2075

页数：18

共 98 条

[11] Versatile performance of a cationic surfactant derived from carbon quantum dots［J］, Acta Phys. Chim. Sinica, 38, 12, (2022)
[12] Fundamentals and developments in fluorescence-guided cancer surgery［J］, Nat. Rev. Clin. Oncol, 19, 1, pp. 9-22, (2022)
[13] WANG K, LIU C Y，, ZHU H C，, Et al., Recent advances in small-molecule fluorescent probes for diagnosis of cancer cells/ tissues［J］, Coord. Chem. Rev, 477, (2023)
[14] FAN J L, WANG J Y，, Et al., An“enhanced PET”-based fluorescent probe with ultrasensitivity for imaging basal and elesclomol-induced HCLO in cancer cells［J］, J. Am. Chem. Soc, 136, 37, pp. 12820-12823, (2014)
[15] WANG H, ZHANG C H，, Et al., Red carbon dots as label-free two-photon fluorescent nanoprobes for imaging of formaldehyde in living cells and zebrafishes［J］, Chin. Chem. Lett, 31, 3, pp. 759-763, (2020)
[16] WANG S X, Et al., Two-photon small-molecule fluorogenic probes for visualizing endogenous nitroreductase activities from tumor tissues of a cancer patient［J］, Adv. Healthc. Mater, 11, 14, (2022)
[17] WANG S X, ZHOU B C, WANG N，, Et al., Mitochondria-targeted fluorescent probe based on vibration-induced emission for real-time monitoring mitophagy-specific viscosity dynamic［J］, Chin. Chem. Lett, 31, 11, pp. 2897-2902, (2020)
[18] ZHANG G B, ZHAO Y F，, PENG B, Et al., A fluorogenic probe based on chelation-hydrolysis-enhancement mechanism for visualizing Zn2+ in Parkinson's disease models［J］, J. Mater. Chem. B, 7, 14, pp. 2252-2260, (2019)
[19] XIN C Q, ZHANG G B, Et al., A mitochondria-targeted two-photon fluorogenic probe for the dual-imaging of viscosity and H2O2 levels in Parkinson's disease models［J］, J. Mater. Chem. B, 7, 27, pp. 4243-4251, (2019)
[20] Optical/electrochemical methods for detecting mitochondrial energy metabolism［J］, Chem. Soc. Rev, 51, 1, pp. 71-127, (2022)

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