Recent Progress in Small-molecule Fuorescent Probes for in situ Imaging and Detection of Enzyme

被引：0
|
作者：
Zhang L. [1 ]
Huang Z. [1 ]
Shen Q. [1 ]
Ju L. [2 ]
Wu Q. [1 ]
Yu C. [1 ]
机构：
[1] School of Flexible Electronics（Future Technologies）, Nanjing Tech University, Nanjing
[2] The Affiliated Cancer Hospital of Nanjing Medical University, Nanjing
来源：
Faguang Xuebao/Chinese Journal of Luminescence | 2023年 / 44卷 / 11期
关键词：
enzyme; in situ imaging; recent progress; small-molecule fluorescent probes;
D O I：
10.37188/CJL.20230128
中图分类号：
学科分类号：
摘要：
Enzyme widely exists in various life activities. Some diseases are closely related to abnormal expression of active enzymes. Accurate detection of enzyme expression levels and in situ imaging provide a powerful basis for diagnosis and treatment of related diseases. Up to now，a large number of detection technologies have been developed，among which the fluorescent technology represented by small-molecule fluorescent probes has advantages such as non-invasive，high sensitivity，low detection limit，fast response time and good biocompatibility. It is favored in the detection of biological enzyme. However，as small-molecule fluorescent probes are used for detection，they tend to diffuse at the active sites of enzyme，resulting in poor spatial and temporal resolution of the probes. Therefore，in order to improve the spatial and temporal resolution of imaging detection and reduce background interference and false positives，the design of in situ imaging has been proposed，which has become one of the research focuses of optical imaging. At present，researchers have reported that a variety of small-molecular fluorescent probes have been used in the design of enzyme in situ imaging and achieved remarkable results. This review will introduce the design strategy of small-molecular fluorescent probes for enzyme detection and the research progress in situ imaging，hoping to provide some inspirations for researchers in this field. © 2023 Chines Academy of Sciences. All rights reserved.
引用
收藏
页码：2057 / 2075
页数：18
相关论文
共 98 条
  • [1] LIU H W, CHEN L L, Et al., Recent progresses in small-molecule enzymatic fluorescent probes for cancer imaging［J］, Chem. Soc. Rev, 47, 18, pp. 7140-7180, (2018)
  • [2] HAN H H, TIAN H J R，, ZANG Y, Et al., Small-molecule fluorescence-based probes for interrogating major organ diseases［J］, Chem. Soc. Rev, 50, 17, pp. 9391-9429, (2021)
  • [3] YANG Y X，, ZHANG L, WANG J M，, Et al., Diagnosis of Alzheimer’s disease and in situ biological imaging via an activatable near-infrared fluorescence probe［J］, Anal. Chem, 94, 39, pp. 13498-13506, (2022)
  • [4] TALUKDAR R, ZHU H Y，, Et al., Release of cathepsin B in cytosol causes cell death in acute pancreatitis ［J］, Gastroenterology, 151, 4, pp. 747-758, (2016)
  • [5] CRUNKHORN S., BACE1 inhibitor reduces β-amyloid production in humans［J］, Nat. Rev. Drug Discov, 16, 1, (2017)
  • [6] WOZNIAK J, Et al., Key metalloproteinase-mediated pathways in the kidney［J］, Nat. Rev. Nephrol, 17, 8, pp. 513-527, (2021)
  • [7] GUO J X, CHEN H Y, YUAN Z W., Advances in organic small molecule fluorescent probes for diagnosis of brain diseases［J］, Shandong Chem. Ind, 51, 22, pp. 105-108, (2022)
  • [8] JIANG X Q, WANG L F，, CARROLL S L，, Et al., Challenges and opportunities for small-molecule fluorescent probes in redox biology applications［J］, Antioxid. Redox Signal, 29, 6, pp. 518-540, (2018)
  • [9] THURBER G M, SCHMIDT M M, WITTRUP K D., Factors determining antibody distribution in tumors［J］, Trends Pharmacol. Sci, 29, 2, pp. 57-61, (2008)
  • [10] GONG Q Y，, AKAKURU O U，, Et al., Research advances in integrated theranostic probes for tumor fluorescence visualization and treatment［J］, Nanoscale, 12, 48, pp. 24311-24330, (2020)
12345678910