CRISPR/Cas-based Nucleic Acid Detection and Its Application in Rapid Detection for Food Safety

被引：0
|
作者：
Zhai B. [2 ]
Cheng N. [3 ]
Wang H. [2 ]
Xu Y. [1 ]
机构：
[1] Faculty of Environment and Life, Beijing University of Technology, Beijing
[2] Henan Railway Food Safety Management Engineering Technology Research Center, Zhengzhou
[3] College of Food Science & Nutritional Engineering, China Agriculture University, Beijing
来源：
Journal of Chinese Institute of Food Science and Technology | 2023年 / 23卷 / 02期
关键词：
clustered regularly interspaced short palindromic repeats (CRISPR); food safety; nucleic acid biosensor; rapid detection;
D O I：
10.16429/j.1009-7848.2023.02.037
中图分类号：
学科分类号：
摘要：
In recent years, rapid detection technology has become one of the most important topics in the scope of food safety. CRISPR/Cas system consists of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas). Due to its superior targetability, CRISPR/Cas system has been widely used in the nucleic acid detection. CRISPR/Cas nucleic acid biosensor has become the research hotspot of rapid detection, because of its high sensitivity, strong specificity and good timeliness, driving the development of rapid detection for food safety. This review summarized the development of CRISPR/Cas system, introducing CRISPR/Cas systems combined with different nucleic acid amplification technologies, and their applications in target detection. Furthermore, the use of CRISPR/Cas nucleic acid biosensors in rapid detection for food safety was discussed, and future research directions was proposed. Overall, this review provides references and ideas for developing novel rapid detection methods utilizing CRISPR/Cas nucleic acid biosensors. © 2023 Chinese Institute of Food Science and Technology. All rights reserved.
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页码：383 / 397
页数：14
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