CRISPR/Cas-based Nucleic Acid Detection and Its Application in Rapid Detection for Food Safety

被引：0
|
作者：
Zhai B. [2 ]
Cheng N. [3 ]
Wang H. [2 ]
Xu Y. [1 ]
机构：
[1] Faculty of Environment and Life, Beijing University of Technology, Beijing
[2] Henan Railway Food Safety Management Engineering Technology Research Center, Zhengzhou
[3] College of Food Science & Nutritional Engineering, China Agriculture University, Beijing
来源：
Journal of Chinese Institute of Food Science and Technology | 2023年 / 23卷 / 02期
关键词：
clustered regularly interspaced short palindromic repeats (CRISPR); food safety; nucleic acid biosensor; rapid detection;
D O I：
10.16429/j.1009-7848.2023.02.037
中图分类号：
学科分类号：
摘要：
In recent years, rapid detection technology has become one of the most important topics in the scope of food safety. CRISPR/Cas system consists of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein (Cas). Due to its superior targetability, CRISPR/Cas system has been widely used in the nucleic acid detection. CRISPR/Cas nucleic acid biosensor has become the research hotspot of rapid detection, because of its high sensitivity, strong specificity and good timeliness, driving the development of rapid detection for food safety. This review summarized the development of CRISPR/Cas system, introducing CRISPR/Cas systems combined with different nucleic acid amplification technologies, and their applications in target detection. Furthermore, the use of CRISPR/Cas nucleic acid biosensors in rapid detection for food safety was discussed, and future research directions was proposed. Overall, this review provides references and ideas for developing novel rapid detection methods utilizing CRISPR/Cas nucleic acid biosensors. © 2023 Chinese Institute of Food Science and Technology. All rights reserved.
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页码：383 / 397
页数：14
相关论文
共 61 条
  • [1] LI Y J, WANG C, ZHU Y B, Et al., Fully integrated graphene electronic biosensor for label -free detection of lead (II) ion based on g-quadruplex structure-switching, Biosensors and Bioelectronics, 89, (2017)
  • [2] ARDUINI F, CINTI S, SCOGNAMIGLIO V, Et al., Nanomaterials in electrochemical biosensors for pesticide detection: Advances and challenges in food analysis, Microchimica Acta, 183, 7, (2016)
  • [3] LIU X B, MARRAKCHI M, XU D W, Et al., Biosensors based on modularly designed synthetic peptides for recognition, detection and live/dead differentiation of pathogenic bacteria, Biosensors and Bioelectronics, 80, (2016)
  • [4] WANG X S, XIONG E H, TIAN T, Et al., Clustered regularly interspaced short palindromic repeats/ cas9 -mediated lateral flow nucleic acid assay, ACS Nano, 14, 2, (2020)
  • [5] MUKAMA O, WU J H, LI Z Y, Et al., An ultrasensitive and specific point -of -care crispr/cas12 based lateral flow biosensor for the rapid detection of nucleic acids, Biosensors and Bioelectronics, 159, (2020)
  • [6] CHEN M, LUO R, LI S, Et al., Based strip for ultrasensitive detection of oscc-associated salivary microrna via CRISPR/Cas12a coupling with is -primer amplification reaction, Analytical Chemistry, 92, 19, (2020)
  • [7] DEVEAU H, GARNEAU J E, MOINEAU S., CRISPR/Cas system and its role in phage -bacteria interactions, Annual Review of Microbiology, 64, 1, (2010)
  • [8] KOONIN E V, MAKAROVA K S, ZHANG F., Diversity, classification and evolution of CRISPR-Cas systems, Current Opinion in Microbiology, 37, (2017)
  • [9] MAKAROVA K S, WOLF Y I, ALKHNBASHI O S, Et al., An updated evolutionary classification of CRISPRøCas systems, Nature Reviews Microbiology, 13, 11, (2015)
  • [10] HSU P D, LANDER E S, ZHANG F., Development and applications of CRISPR-Cas9 for genome engineering, Cell, 157, 6, (2014)
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